PXD063443 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | EP3 deletion in macrophages aggravates diet-induced obesity in mice by suppressing SPARC |
Description | Macrophages are primary immune cells involved in obesity-triggered chronic low-grade inflammation in adipose tissues. Prostaglandin (PG) E2, mainly generated from macrophages, can regulate adipose tissue remodeling. Here, we observed that PGE2 receptor subtype 3 (EP3) was remarkably downregulated in adipose tissue macrophages from high-fat diet (HFD)-fed mice and patients with obesity. Notably, macrophage-specific deletion of EP3 exacerbated HFD-induced obesity in mice, whereas EP3α isoform overexpression in macrophages alleviated obesity phenotype in HFD-fed mice. EP3 deficiency suppressed anti-adipogenic secreted protein acidic and rich in cysteine (SPARC) secretion in macrophages. SPARC deletion in macrophages abrogated the protection of EP3α-overexpression against HFD-induced obesity in mice. Mechanistically, EP3 activation promoted SPARC expression by suppressing DNA methylation in macrophages through the PKA/Sp1/Dnmt1/3a signaling cascade. EP3 agonist treatment ameliorates HFD-induced obesity in mice. Thus, EP3 inhibits adipogenesis through promoting macrophage releasing SPARC and may serve as a therapeutic target for managing diet-induced obesity. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-25 |
AnnouncementXML | Submission_2025-05-25_02:38:00.893.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | wenlong shang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-04-29 06:24:16 | ID requested | |
⏵ 1 | 2025-05-25 02:38:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Macrophage,Obesity, SPARC, E-prostanoid 3 receptor |
Contact List
Ying Yu |
contact affiliation | Department of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Experimental Hematology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. |
contact email | yuying@tmu.edu.cn |
lab head | |
wenlong shang |
contact affiliation | Tianjin Medical University |
contact email | shangwenlong@tmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063443
- Label: PRIDE project
- Name: EP3 deletion in macrophages aggravates diet-induced obesity in mice by suppressing SPARC