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PXD063443

PXD063443 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEP3 deletion in macrophages aggravates diet-induced obesity in mice by suppressing SPARC
DescriptionMacrophages are primary immune cells involved in obesity-triggered chronic low-grade inflammation in adipose tissues. Prostaglandin (PG) E2, mainly generated from macrophages, can regulate adipose tissue remodeling. Here, we observed that PGE2 receptor subtype 3 (EP3) was remarkably downregulated in adipose tissue macrophages from high-fat diet (HFD)-fed mice and patients with obesity. Notably, macrophage-specific deletion of EP3 exacerbated HFD-induced obesity in mice, whereas EP3α isoform overexpression in macrophages alleviated obesity phenotype in HFD-fed mice. EP3 deficiency suppressed anti-adipogenic secreted protein acidic and rich in cysteine (SPARC) secretion in macrophages. SPARC deletion in macrophages abrogated the protection of EP3α-overexpression against HFD-induced obesity in mice. Mechanistically, EP3 activation promoted SPARC expression by suppressing DNA methylation in macrophages through the PKA/Sp1/Dnmt1/3a signaling cascade. EP3 agonist treatment ameliorates HFD-induced obesity in mice. Thus, EP3 inhibits adipogenesis through promoting macrophage releasing SPARC and may serve as a therapeutic target for managing diet-induced obesity.
HostingRepositoryPRIDE
AnnounceDate2025-05-25
AnnouncementXMLSubmission_2025-05-25_02:38:00.893.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterwenlong shang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-29 06:24:16ID requested
12025-05-25 02:38:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Macrophage,Obesity, SPARC, E-prostanoid 3 receptor
Contact List
Ying Yu
contact affiliationDepartment of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Experimental Hematology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
contact emailyuying@tmu.edu.cn
lab head
wenlong shang
contact affiliationTianjin Medical University
contact emailshangwenlong@tmu.edu.cn
dataset submitter
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Dataset FTP location
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