PXD063426

PXD063426 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric identification of colorectal cancer cells
Description	Immunoprecipitation experiments were carried out to enrich TRIM24 following the lysis of DLD1 cells. The sample was added to 100 µL of 100 mM NH4HCO3, followed by trypsin (10 ng/µL) digestion at 37°C for 17 hours. Then, the sample was centrifuged at 12,000 g for 30 min, and the supernatant was freeze-dried, desalted, and freeze-dried again. Subsequently, 15 µL of 0.1% FA solution was added to dissolve the freeze-dried peptide. After centrifugation at 12,000 g for 5 min, the supernatant was transferred to the sample vial for mass spectrometry (Q Exactive HFX) analysis. The pFind (3.1.5) database search was performed, with Oxidation (M), Acetyl (Protein N-term), and Phospho (STY) set as variable modifications, and the false discovery rate of protein identification was controlled to be less than 1%.
HostingRepository	iProX
AnnounceDate	2025-04-29
AnnouncementXML	Submission_2025-04-29_00:03:44.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 00:03:24	ID requested
⏵ 1	2025-04-29 00:03:45	announced

Dataset with its publication pending

submitter keyword: Colorectal cancer, Immunoprecipitation, TRIM24

Kai Fu
contact affiliation	Xiangya Hospital, Central South University
contact email	fu_kai@csu.edu.cn
lab head
Ya Wang
contact affiliation	The First Affiliated Hospital, University of South China
contact email	aya520123@163.com
dataset submitter

iProX dataset URI