PXD063423

PXD063423 is an original dataset announced via ProteomeXchange.

Title	Photocatalytic Proximity Labeling Coupled with Cross-linking for Deciphering of the Lysosome Proteome in Living Cells
Description	Lysosomes are essential organelles that play vital roles in the degradation and recycling of a variety of biomolecules. To fully understand their molecular mechanisms, high spatiotemporal identification of the lysosome proteome in living cells is critical. Although photocatalytic proximity labeling is a powerful tool for profiling subcellular proteome, it often struggles with low labeling efficiency, particularly for low-abundance proteins. To overcome this, we introduced a novel strategy that integrates photocatalytic proximity labeling with cross-linking. This approach enabled difficult-to-label proteins to be linked to already labeled ones via cross-linking, thereby expanding the scope of pro-tein identification. Using this strategy, we successfully identified 238 lysosome-annotated proteins in living HeLa cells, a substantial in-crease compared to method without cross-linking (238 vs 197). The developed strategy enhanced lysosomal proteomic coverage via a high-precision integration platform, allowing systematic identification of both membrane-associated and luminal proteins within lysosome, thereby providing mechanistic insights into subcellular biology and unlocking new frontiers in cellular research.
HostingRepository	iProX
AnnounceDate	2025-04-29
AnnouncementXML	Submission_2025-04-28_23:48:29.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 23:48:14	ID requested
⏵ 1	2025-04-28 23:48:29	announced

Dataset with its publication pending

submitter keyword: Photocatalytic Proximity Labeling, Cross-linking, Lysosome Proteome

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
He Wang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	crane_wanghe@dicp.ac.cn
dataset submitter

iProX dataset URI