PXD063410

PXD063410 is an original dataset announced via ProteomeXchange.

Title	The type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic E. coli
Description	RNA-binding proteins (RBPs) play key roles in regulating virulence gene expression in pathogenic, enterohaemorrhagic E. coli (EHEC). Under virulence-inducing conditions total RNA associated protein purification (TRAPP) identified 443 RNA-associated proteins in EHEC included 35 encoded within pathogen-specific regions of the genome. RNA-binding was confirmed for a sub-set that including RhsFI. In vivo RNA targets for RhsFI included regulatory small RNAs (sRNAs) encoded in 3’ UTRs and we find that sRNA stability is affected in a deletion background. The rhsF-rhsFI locus encodes effector and immunity proteins for the type 6 secretion system (T6SS). T6SS expression is repressed in EHEC, but internal transcription initiation within rhsF generates a sub-genic transcript encoding the toxin domain (RhsFint) and downstream RhsFI. Modelling predicted that RhsFint can dimerise with RhsFI. Deletion of rhsFint rhsFI alters the expression of multiple operons in which embedded sRNAs are direct RhsFI targets. Loss of RhsFint-RhsFI dramatically reduced adhesion to bovine cells in vitro, altered cell morphology and increased acid resistance. Our results indicate that the T6SS immunity protein, RhsFI, binds sRNA in vivo and disrupts the sRNA regulatory network in EHEC to promote host colonisation.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:54:34.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christos Spanos
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 15:10:16	ID requested
⏵ 1	2026-02-22 16:54:35	announced

Zammit T, Sy B, Shchepachev V, Mediati DG, Poudyal P, Norris D, Bang S, Alquethamy S, Wu W, Ullah A, McAteer S, Rowell K, Michie K, Gally D, Tollervey D, Tree J, coli. Nucleic Acids Res, 53(14):(2025) [pubmed]

10.1093/nar/gkaf749;

submitter keyword: Label-free Quantitation, small RNA,RNA-binding proteins, Data Dependent Acquisition, E.coli

David Tollervey
contact affiliation	University of Edinburgh
contact email	d.tollervey@ed.ac.uk
lab head
Christos Spanos
contact affiliation	University of Edinburgh
contact email	spanos.christos@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063410
     1. Label: PRIDE project
     2. Name: The type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic E. coli