PXD063409

PXD063409 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins
Description	The Toxoplasma gondii cytoskeleton is a highly organized structure essential for parasite motility, replication, and host cell invasion. To identify its components, a highly enriched fraction of tachyzoite cytoskeletons was obtained and quantitatively analyzed by mass spectrometry (LC/ESI/HDMSE). We identified 623 proteins classified into 18 functional groups, including 21 IMC proteins, 26 cytoskeleton proteins, and 42 uncharacterized proteins. A comprehensive bioinformatic analysis was conducted to assess protein abundance (fmol), antigenicity, accessibility, interactome, and homology, with the aim of identifying immunogenic targets. Among the top vaccine candidates were GRA12, IMC1, ROP8, and IMC4, with ROP8 emerging as the most promising based on epitope prediction.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:08:11.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karina Araujo
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Eukaryota; NCBI TaxID: NCBITaxon:2759;
ModificationList	amidated residue; phosphorylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 15:06:20	ID requested
⏵ 1	2025-08-03 16:08:12	announced

Araujo-Ruiz K, L, ó, pez-Flores DI, Mart, í, nez-Mun, é MK, Garc, í, a-S, á, nchez BY, Ram, í, rez-Flores CJ, Sandoval-Rodr, í, guez FE, R, í, os-Castro E, Mondrag, ó, n-Castel, á, n ME, Gonz, á, lez-Pozos S, Mondrag, ó, n-Flores R, Quantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins. J Proteomics, 321():105509(2025) [pubmed]

10.1016/j.jprot.2025.105509;

submitter keyword: Toxoplasma gondii, Quantitative Mass Spectrometry, Immunogenic peptides, Cytoskeleton

Ricardo Mondragón-Flores
contact affiliation	Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav). Av. IPN No. 2508, Ciudad de México, C.P. 07360.
contact email	rmflores@cinvestav.mx
lab head
Karina Araujo
contact affiliation	PhD Student
contact email	karina.araujo@cinvestav.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063409

PRIDE project URI

• PRIDE
  1. PXD063409
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins