PXD063391

PXD063391 is an original dataset announced via ProteomeXchange.

Title	Architecture and Function of Holocentric CENP-A-Independent Inner Kinetochores
Description	Kinetochores are essential macromolecular complexes anchoring chromosomes to the mitotic spindle, ensuring faithful cell division. Despite their critical role, the structural organization of kinetochores over large centromeric regions and across diverse species remains poorly understood. We present the inner kinetochore (CCAN) structure of the silkmoth Bombyx mori, an insect without the canonical centromeric CENP-A gene and with chromosome-wide centromeric activity (holocentric). Our analyses reveal a ring-shaped complex with structural parallels to the known human and yeast kinetochores. Notably, B. mori CCAN incorporates four previously uncharacterized proteins, Centromeric Subunits 1-4, which have unexpected evolutionary relationships to the outer kinetochore Dam1/DASH complex. We demonstrate that CCAN binds DNA as a distinctive head-to-head dimer, folding the DNA into a loop and generating an alternative point-centromere-like architecture poised for chromosome segregation. Our work establishes this self-contained CCAN dimer as a key structural unit that forms the basis of a holocentric organization and suggests that large-scale centromere architectures can emerge from the modular arrangement of such discrete kinetochore units.
HostingRepository	PRIDE
AnnounceDate	2026-04-20
AnnouncementXML	Submission_2026-04-20_05:33:14.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Spodoptera frugiperda; NCBI TaxID: NEWT:7108;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 06:15:10	ID requested
⏵ 1	2026-04-20 05:33:15	announced

Dataset with its publication pending

submitter keyword: Kinetochore, Insects, Structure

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063391

PRIDE project URI

• PRIDE
  1. PXD063391
     1. Label: PRIDE project
     2. Name: Architecture and Function of Holocentric CENP-A-Independent Inner Kinetochores