PXD063388

PXD063388 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicle properties and functions are defined by cell fitness status
Description	Recent research has focused on understanding the influence of dying cells on immune function and the surrounding microenvironment. Apoptotic cells release a variety of extracellular vesicles (EVs), such as exosomes and microvesicles, and form apoptotic bodies through membrane blebbing. Despite increasing interest in EVs, the effects of apoptotic cell- versus live cell-derived EVs, have been poorly studied. Here, via transmission electron microscopy, nanoparticle tracking analysis, proteomics, and flow cytometry, we characterise the distinct features of EVs from live versus apoptotic cells. Using a model of Schistosoma mansoni infection, characterised by the presence of dying cells due to parasite egg accumulation, we demonstrated that adoptive transfer of apoptotic T cell-derived EVs attenuates liver pathology, in contrast to EVs from live T cells. Analysis of the transcriptomic profile of target macrophages and in vitro functional assays revealed that apoptotic cell-derived EVs activate NO-related pathways, which modulate macrophage function. Furthermore, these EVs specifically promoted fibroblast-mediated wound healing in vitro. Collectively, our findings highlight that cell fitness influences EV properties and their immune regulatory function. Investigating the differences between EVs from living and apoptotic cells is essential for advancing our understanding of immune regulation and optimising the development of EV-based therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_16:23:59.449.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonia Elisabeth Gocke
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 05:21:38	ID requested
⏵ 1	2026-03-01 16:24:00	announced

10.1002/jex2.70115;

Leyk S, Liebold I, Lanzloth C, Richardt U, Kux JM, Kilian C, Moritz M, Gocke A, Meyer S, H, ö, hn K, Honecker B, Haas H, B, ö, ttcher M, Weidemann S, Kind S, Schl, ü, ter H, Metwally NG, Adlung L, S, á, ez PJ, Jacobs T, Ruckdeschel K, Bosurgi L, Extracellular Vesicle Properties and Functions Are Defined by the Originating Cell's Fitness Status. J Extracell Biol, 5(2):e70115(2026) [pubmed]

submitter keyword: Extracellular vesicles, Bottom-up Proteomics, LC-MS/MS

Hartmut Schlüter
contact affiliation	Section/Core Facility Mass Spectrometry and Proteomics, Center for Diagnostics, University Medical Center Hamburg−Eppendorf (UKE), Hamburg, Germany
contact email	hschluet@uke.de
lab head
Antonia Elisabeth Gocke
contact affiliation	Section of Mass Spectrometric Proteomics, University Medical Center Hamburg Eppendorf, Hamburg, Germany Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg Eppendorf, Hamburg, Germany
contact email	an.gocke@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD063388

PRIDE project URI

• PRIDE
  1. PXD063388
     1. Label: PRIDE project
     2. Name: Extracellular vesicle properties and functions are defined by cell fitness status