PXD063369

PXD063369 is an original dataset announced via ProteomeXchange.

Title	Detecting protein higher-order structural changes using kinase as a phospho-labeler
Description	We developed an in vitro kinase-based approach to detect proteome-wide protein conformational changes. By combining residue-specific phosphorylation with phosphopeptide enrichment, this method enables sensitive, site-specific structural profiling. We demonstrated that phosphorylation efficiency reflected structural differences and revealed conformational changes upon RNA digestion across the proteome.
HostingRepository	jPOST
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_08:00:05.017.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Asato Maeda
SpeciesList	scientific name: Equus caballus; NCBI TaxID: 9796; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; TMTpro; TMTpro
Instrument	Q Exactive; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-27 04:56:06	ID requested
⏵ 1	2026-04-26 08:00:05	announced

Dataset with its publication pending

submitter keyword: Structural proteomics, Protein structure, Mass spectrometry, In vitro kinase reaction, Phosphorylation, Phosphopeptide enrichment, Phosphoproteomics

Yasushi Ishihama
lab head
Asato Maeda
contact affiliation	Kyoto University
dataset submitter

jPOST dataset URI

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