PXD063357
PXD063357 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Increasing mass spectrometry throughput using time-encoded sample multiplexing |
| Description | Liquid chromatography-mass spectrometry (LC-MS) can enable precise and accurate quantification of analytes at high-sensitivity, but the rate at which samples can be analyzed remains limiting. Throughput can be increased by multiplexing samples in the mass domain with plexDIA, yet multiplexing along one dimension will only linearly scale throughput with plex. To enable combinatorial-scaling of proteomics throughput we developed a complementary multiplexing strategy in the time domain, termed `timePlex'. timePlex staggers and overlaps the separation periods of individual samples. This strategy is orthogonal to isotopic multiplexing, which enables combinatorial multiplexing in mass and time domains when paired together and thus multiplicatively increased throughput. We demonstrate this with 3-timePlex and 3-plexDIA, enabling the multiplexing of 9 samples per LC-MS run, and 3-timePlex and 9-plexDIA exceeding 500 samples / day with a combinatorial 27-plex. Crucially, timePlex supports sensitive analyses, including of single cells. These results establish timePlex as a methodology for label-free multiplexing and for combinatorially scaling the throughput of LC-MS proteomics. We project this combined approach will eventually enable an increase in throughput exceeding 1,000 samples / day. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-05-22 |
| AnnouncementXML | Submission_2025-05-22_10:14:08.303.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jason Derks |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | mTRAQ |
| Instrument | Orbitrap Exploris 240; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-04-26 11:57:52 | ID requested | |
| ⏵ 1 | 2025-05-22 10:14:08 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: DIA, plexDIA, timePlex, Single-cell, DatasetType:Proteomics |
Contact List
| Nikolai Slavov | |
|---|---|
| contact affiliation | Parallel Squared Technology Institute |
| contact email | nslavov@parallelsq.org |
| lab head | |
| Jason Derks | |
| contact affiliation | Northeastern University |
| contact email | derks.j@northeastern.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097736/ |
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