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PXD063357

PXD063357 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIncreasing mass spectrometry throughput using time-encoded sample multiplexing
DescriptionLiquid chromatography-mass spectrometry (LC-MS) can enable precise and accurate quantification of analytes at high-sensitivity, but the rate at which samples can be analyzed remains limiting. Throughput can be increased by multiplexing samples in the mass domain with plexDIA, yet multiplexing along one dimension will only linearly scale throughput with plex. To enable combinatorial-scaling of proteomics throughput we developed a complementary multiplexing strategy in the time domain, termed `timePlex'. timePlex staggers and overlaps the separation periods of individual samples. This strategy is orthogonal to isotopic multiplexing, which enables combinatorial multiplexing in mass and time domains when paired together and thus multiplicatively increased throughput. We demonstrate this with 3-timePlex and 3-plexDIA, enabling the multiplexing of 9 samples per LC-MS run, and 3-timePlex and 9-plexDIA exceeding 500 samples / day with a combinatorial 27-plex. Crucially, timePlex supports sensitive analyses, including of single cells. These results establish timePlex as a methodology for label-free multiplexing and for combinatorially scaling the throughput of LC-MS proteomics. We project this combined approach will eventually enable an increase in throughput exceeding 1,000 samples / day.
HostingRepositoryMassIVE
AnnounceDate2025-05-22
AnnouncementXMLSubmission_2025-05-22_10:14:08.303.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJason Derks
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationListmTRAQ
InstrumentOrbitrap Exploris 240; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-26 11:57:52ID requested
12025-05-22 10:14:08announced
Publication List
no publication
Keyword List
submitter keyword: DIA, plexDIA, timePlex, Single-cell, DatasetType:Proteomics
Contact List
Nikolai Slavov
contact affiliationParallel Squared Technology Institute
contact emailnslavov@parallelsq.org
lab head
Jason Derks
contact affiliationNortheastern University
contact emailderks.j@northeastern.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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