PXD063320

PXD063320 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of gemcitabine-resistant (GemR) versus paired control human pancreatic cancer cells (PCCs)
Description	In search of potential markers of treatment response, comparative LC-MS/MS-based proteomic analysis of GemR and paired control PCCs was performed. It includes four different PCC lines including three primary - PCC-1 (c1), PCC-2 (c2), PCC-7 (c3) and Mia PaCa-2 (c4). Cells were maintained in DMEM supplemented with 10% FBS, with or without gemcitabine (50-150 nM) for upto 40 passages. Cell lysates in triplicate from GemR and paired control cells collected at six different passages (p10, p20, p25, p30, p35, and p40) during treatment period were analyzed using LC-MS/MS
HostingRepository	PRIDE
AnnounceDate	2025-11-04
AnnouncementXML	Submission_2025-11-04_12:33:15.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ingrid Maria Erika Ekman Stensland
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-25 03:36:11	ID requested
⏵ 1	2025-11-04 12:33:16	announced

10.3390/proteomes13040048;

Amrutkar M, Li Y, Finstadsveen AV, Verbeke CS, Gladhaug IP, Proteomic Characterization of Primary Human Pancreatic Cancer Cell Lines Following Long-Term Exposure to Gemcitabine. Proteomes, 13(4):(2025) [pubmed]

submitter keyword: proteome profiling,Pancreatic cancer, gemcitabine resistance, LC-MS/MS

Tuula A. Nyman
contact affiliation	The Proteomics Core Facility (PCF) at Oslo University Hospital
contact email	t.a.nyman@medisin.uio.no
lab head
Ingrid Maria Erika Ekman Stensland
contact affiliation	Oslo University hospital
contact email	maria.stensland@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD063320

PRIDE project URI

• PRIDE
  1. PXD063320
     1. Label: PRIDE project
     2. Name: Proteomic analysis of gemcitabine-resistant (GemR) versus paired control human pancreatic cancer cells (PCCs)