PXD063288

PXD063288 is an original dataset announced via ProteomeXchange.

Title	A light-resuming strategy as a screening method for Sec61 inhibitors down-modulating PD-L1 expression
Description	The perturbation of protein translocation into the secretory pathway using Sec61 translocon inhibitors is a novel and promising strategy for tackling many pathological situations, including cancer and viral infections. However, a highly sensitive and direct screening platform for selecting Sec61 inhibitors is unavailable. Here, we develop a new “resuming luminescence upon translocation interference” (RELITE) assay capable of selecting Sec61 inhibitors in a single round of screening. This assay exploits the inactivation of firefly luciferase, once translocated into the endoplasmic reticulum (ER), and the possibility of diverting and “re-lighting” luciferase into the cytosol by a Sec61 inhibitor. Using this method, we selected small molecules capable of hampering the protein expression of the PD-L1 immune checkpoint by interfering with its ER translocation and delivering it for degradation. In conclusion, our screening method will greatly facilitate the selection of Sec61 inhibitors for down-modulating the expression of many disease-relevant proteins.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-04_10:08:08.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Massimo D'Agostino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-24 08:37:28	ID requested
⏵ 1	2025-08-04 10:08:08	announced

10.1038/S41467-025-62439-W;

submitter keyword: Sec61, secretory pathway,PD-L1

Massimo D
contact affiliation	University of Naples Federico II
contact email	massimo.dagostino@unina.it
lab head
Massimo D'Agostino
contact affiliation	Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II
contact email	massimo.dagostino@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063288

PRIDE project URI

• PRIDE
  1. PXD063288
     1. Label: PRIDE project
     2. Name: A light-resuming strategy as a screening method for Sec61 inhibitors down-modulating PD-L1 expression