PXD063272 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ |
Description | Amyloid aggregation is associated with neurodegenerative disease and its modulation is a focus of drug development. We developed a pipeline based on limited proteolysis-mass spectrometry (LiP-MS) to probe the mechanism of action of anti-amyloidogenic compounds. Our approach identifies putative interaction sites, can probe compound interactions with specific target conformations in complex extracts, and identifies off-targets. We analysed interactions of six anti-amyloidogenic compounds and the amyloid binder Thioflavin T with different structural forms of the Parkinson’s disease protein α-Synuclein. AC Immune compound 2 interacted with α-Synuclein in vitro, in intact neurons and in neuronal lysates, reduced neuronal α-Synuclein levels in a seeded model, and had protective effects. EGCG, Baicalein, ThT and doxycycline interacted with α-Synuclein in vitro but not substantially in mammalian cell lysates, with many additional putative targets. Our modular pipeline will enable in situ screening of compounds against any amyloid aggregates and mechanistic studies of compound action. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-26 |
AnnouncementXML | Submission_2025-05-25_20:29:16.232.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tetiana Serdiuk |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-04-24 00:41:44 | ID requested | |
⏵ 1 | 2025-05-25 20:29:17 | announced | |
Publication List
10.1038/s41531-025-00966-5; |
Stalder P, Serdiuk T, Ghosh D, Fleischmann Y, Ait-Bouziad N, Quast JP, Malinovska L, Ouared A, Davranche A, Haenseler W, Boudou C, Tsika E, St, ö, hr J, Melki R, Riek R, de Souza N, Picotti P, An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ. NPJ Parkinsons Dis, 11(1):122(2025) [pubmed] |
Keyword List
submitter keyword: alpha-synuclein, anti-amyloidogenic compounds,LiP-MS |
Contact List
Paola Picotti |
contact affiliation | Institute of Molecular Systems Biology, ETH Zurich |
contact email | picotti@imsb.biol.ethz.ch |
lab head | |
Tetiana Serdiuk |
contact affiliation | Institute of Molecular Systems Biology, ETH Zurich, Switzerland |
contact email | tserdiuk@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063272
- Label: PRIDE project
- Name: An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ