PXD063249

PXD063249 is an original dataset announced via ProteomeXchange.

Title	Hydrodynamically-Assembled Multicellular System towards structural self-organization and functional crosstalk
Description	In vitro models are widely used in fundamental research and high-throughput drug discovery. Particularly the sensory neurons dissociated from dorsal root ganglion (DRG) are cultured in monolayer to study the pain associated biology. The problem of monolayer culture is that the naïve multicellular architecture is deprived. The impact of multicellular assembly on biology remains elusive. Also, biofabrication of multicellular system usually depends on a complex scaffold design to support the aggregate which is difficult to scale up. The scaffold-cell interaction may confound the in vitro testing result. Here, we present a scaffold-free assembly using hydrodynamic forces. Cells are assembled in normal culture medium into a precise geometry within two minutes. During culture, the DRG cells self-organize into a 2.5 ~ 3-dimensional architecture. Cells binds to each other to maintain their shape and mechanobiology. We demonstrate interneuron crosstalk in the multicellular system via the synchronization of calcium signals and the cluster-wise expression of neuronal activation markers. Thus, multicellular architecture determines the cell shape and functional crosstalk. This evidence pinpoints the multicellular principle of in vitro models. The scaffold-free assembling is a simple tool to improve the robustness of in vitro model in predicting in vivo biology, which may benefit the innovation in pain medicine.
HostingRepository	PRIDE
AnnounceDate	2026-05-06
AnnouncementXML	Submission_2026-05-06_02:50:45.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcello Manfredi
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-23 08:55:49	ID requested
⏵ 1	2026-05-06 02:50:46	announced

10.1016/J.CELBIO.2026.100489;

submitter keyword: In vitro models, 3D arc hitecture, Interneurons cross-talk, Sensory neurons, Scaffold-free assembly

Marcello Manfredi
contact affiliation	University of Piemonte Orientale
contact email	marcello.manfredi@uniupo.it
lab head
Marcello Manfredi
contact affiliation	University of Eastern Piedmont
contact email	marcello.manfredi@uniupo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD063249

PRIDE project URI

• PRIDE
  1. PXD063249
     1. Label: PRIDE project
     2. Name: Hydrodynamically-Assembled Multicellular System towards structural self-organization and functional crosstalk