PXD063200

PXD063200 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling of dense core vesicle protein VMAT
Description	Neuropeptide-containing dense core vesicles (DCVs) are generated in the neuronal cell body and circulate throughout the axonal arbor to supply distal release sites. This circulation depends on the anterograde kinesin-1 and kinesin-3 motors and the retrograde dynein-dynactin motor, however the adaptor proteins involved are poorly understood. In addition to their luminal neuropeptide cargo DCVs also contain specific transmembrane proteins including the vesicular monoamide transporter (VMAT). To identify potential components of DCV motor adaptor complexes in Drosophila neurons we performed proximity labeling based on a pan-neuronally expressed TurboID-VMAT chimera, as well as a free cytosolic TurboID used as a negative control. This approach may also shed light on the trafficking pathways involved in sorting VMAT and other membrane proteins to DCVs.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_16:42:31.344.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 09:55:37	ID requested
⏵ 1	2026-01-11 16:42:32	announced

Lund VK, Chirco A, Caliari M, Larsen AH, Tang KT, Gether U, Madsen KL, Wierer M, Kjaerulff O, A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles. J Cell Biol, 225(3):(2026) [pubmed]

10.1083/jcb.202507071;

submitter keyword: synaptic vesicles, VMAT, neurons, axonal transport,Neuropeptide-containing dense core vesicles, Drosophila

Ole Kjaerulff
contact affiliation	Department of Neuroscience, University of Copenhagen
contact email	okjaerulff@sund.ku.dk
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063200

PRIDE project URI

• PRIDE
  1. PXD063200
     1. Label: PRIDE project
     2. Name: Proximity labeling of dense core vesicle protein VMAT