PXD063181

PXD063181 is an original dataset announced via ProteomeXchange.

Title	A proteomic study of the dual oncogenic and tumor- suppressive roles of SIRT3 in lung and breast cancer cell lines
Description	The mitochondria play a crucial role in metabolism and energy production by generating adenosine triphosphate (ATP) through oxidative phosphorylation. Additionally, they are involved in maintaining intracellular calcium levels, facilitate nuclear communication, detoxify reactive oxygen species (ROS), and regulate apoptosis. While not all protein regulation pathways are fully understood, mitochondrial protein acetylation is known to have an inhibitory effect, and the SIRT3 deacetylase plays a key role in this process, particularly in relation to cancer development. Our study on lung cancer cells (A549) and breast cancer cells (MCF7) revealed significant regulation through the processes of protein acetylation and deacetylation, with distinct effects from SIRT3 inhibition observed in each cell type. In A549 cells, inhibiting SIRT3 appears to impede cancer progression. In contrast, in MCF7 cells, SIRT3 activates unfavorable cancer-related pathways, suggesting that SIRT3 may have an oncogenic role in A549 and a suppressive function in MCF7. These findings highlight SIRT3 as a promising therapeutic target in cancer treatment.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-15_17:54:04.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARISOL AYALA REYES
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 02:52:33	ID requested
⏵ 1	2026-02-15 17:54:05	announced

10.3390/ijms27031325;

Ayala Reyes M, Fern, á, ndez Coto DL, Alonso Bastida R, Marko-Varga G, Gil J, Encarnaci, ó, n-Guevara S, A Proteomic Study of the Dual Oncogenic and Tumor-Suppressive Roles of SIRT3 in Lung and Breast Cancer Cell Lines. Int J Mol Sci, 27(3):(2026) [pubmed]

submitter keyword: mitochondria

cancer

post-translational modification

acetylation

sirtuin

metabolism

oxidative phosphorylation

Sergio Encarnación Guevara
contact affiliation	Programa de Genómica Funcional de Procariotes, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, México.
contact email	encarnac@ccg.unam.mx
lab head
MARISOL AYALA REYES
contact affiliation	UNAM
contact email	mar.ayala1863@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063181

PRIDE project URI

• PRIDE
  1. PXD063181
     1. Label: PRIDE project
     2. Name: A proteomic study of the dual oncogenic and tumor- suppressive roles of SIRT3 in lung and breast cancer cell lines