PXD063179

PXD063179 is an original dataset announced via ProteomeXchange.

Title	Covalent targeting leads to the development of LIMK1 isoform-selective inhibitors
Description	SM311 is a covalent LIMK1 inhibitor. To verify the specificity of SM311 towards LIMK1, a chemoproteomic competition experiment was set up with a biotin adduct of SM311, namely SM429. This adduct was immobilised on Streptactin beads and used to enrich proteins binding to SM429. Preincubation with an excess of SM311 was used as control to outcompete and block the binding of SM429. In the competition setup we show that LIMK1 is depleted if SM311 is added in excess but also identified two additional kinase off-targets that required further profiling.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:19:47.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Gstaiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 01:57:28	ID requested
⏵ 1	2025-08-03 16:19:48	announced

Mandel S, Hanke T, Prendiville N, Baena-Nuevo M, Berger LM, Farges F, Schwalm MP, Berger BT, Kraemer A, Elson L, Saraswati H, Abdul Azeez KR, Dederer V, Mathea S, Corrionero A, Alfonso P, Keller S, Gstaiger M, Krause DS, M, ü, ller S, R, ö, hm S, Knapp S, Covalent Targeting Leads to the Development of a LIMK1 Isoform-Selective Inhibitor. J Med Chem, 68(14):15026-15049(2025) [pubmed]

10.1021/acs.jmedchem.5c01204;

submitter keyword: chemoproteomics, kinase, LIMK1,human

Matthias Gstaiger
contact affiliation	ETH Zürich Institute of Molecular Systems Biology Otto-Stern-Weg 3 8093 Zürich Switzerland
contact email	gstaigem@ethz.ch
lab head
Matthias Gstaiger
contact affiliation	Institute of Molecular Systems Biology, ETH
contact email	gstaigem@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063179

PRIDE project URI

• PRIDE
  1. PXD063179
     1. Label: PRIDE project
     2. Name: Covalent targeting leads to the development of LIMK1 isoform-selective inhibitors