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PXD063173

PXD063173 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe proteomics data for Increased matrix stiffness promotes fibrogenesis of hepatic stellate cells through AP-1-induced chromatin priming
DescriptionMatrix stiffness has significant effects on cell behavior, however, less is known regarding the epigenomic and transcriptional regulation underling the effect of matrix stiffness on cells. In this study, we use an in vitro system to assess the phenotypic shifts of hepatic stellate cells (HSCs) following changes in matrix stiffness, and integrate multi-omics with imaging and biochemical assays to investigate the molecular mechanisms. We show that cells cultured on a stiff matrix display more accessible chromatin sites, which consist of primed chromatin regions that become more accessible prior to the upregulation of nearby genes. These regions are enriched in fibrosis-associated genes that function in cytoskeletal organization and response to mechanical stimuli. We also identify activation of p-JUN in response to the stiff matrix and promoting phenotypic shifts. The identified chromatin accessibility-dependent effect of matrix stiffness may be responsible for various fibrotic diseases and provide insight into
HostingRepositoryiProX
AnnounceDate2025-04-19
AnnouncementXMLSubmission_2025-04-21_21:30:44.076.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenxue Zhao
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-21 21:29:45ID requested
12025-04-21 21:30:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: hepatic stellate cell
Contact List
Yujie Sun
contact affiliationPeking University
contact emailsun_yujie@pku.edu.cn
lab head
Wenxue Zhao
contact affiliationPeking University
contact emailzhaowenxue@stu.pku.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI