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PXD063173

PXD063173 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The proteomics data for Increased matrix stiffness promotes fibrogenesis of hepatic stellate cells through AP-1-induced chromatin priming
Description	Matrix stiffness has significant effects on cell behavior, however, less is known regarding the epigenomic and transcriptional regulation underling the effect of matrix stiffness on cells. In this study, we use an in vitro system to assess the phenotypic shifts of hepatic stellate cells (HSCs) following changes in matrix stiffness, and integrate multi-omics with imaging and biochemical assays to investigate the molecular mechanisms. We show that cells cultured on a stiff matrix display more accessible chromatin sites, which consist of primed chromatin regions that become more accessible prior to the upregulation of nearby genes. These regions are enriched in fibrosis-associated genes that function in cytoskeletal organization and response to mechanical stimuli. We also identify activation of p-JUN in response to the stiff matrix and promoting phenotypic shifts. The identified chromatin accessibility-dependent effect of matrix stiffness may be responsible for various fibrotic diseases and provide insight into
HostingRepository	iProX
AnnounceDate	2025-04-19
AnnouncementXML	Submission_2025-04-21_21:30:44.076.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxue Zhao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-21 21:29:45	ID requested
⏵ 1	2025-04-21 21:30:44	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: hepatic stellate cell

Contact List

Yujie Sun
contact affiliation	Peking University
contact email	sun_yujie@pku.edu.cn
lab head
Wenxue Zhao
contact affiliation	Peking University
contact email	zhaowenxue@stu.pku.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI