PXD063173
PXD063173 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteomics data for Increased matrix stiffness promotes fibrogenesis of hepatic stellate cells through AP-1-induced chromatin priming |
Description | Matrix stiffness has significant effects on cell behavior, however, less is known regarding the epigenomic and transcriptional regulation underling the effect of matrix stiffness on cells. In this study, we use an in vitro system to assess the phenotypic shifts of hepatic stellate cells (HSCs) following changes in matrix stiffness, and integrate multi-omics with imaging and biochemical assays to investigate the molecular mechanisms. We show that cells cultured on a stiff matrix display more accessible chromatin sites, which consist of primed chromatin regions that become more accessible prior to the upregulation of nearby genes. These regions are enriched in fibrosis-associated genes that function in cytoskeletal organization and response to mechanical stimuli. We also identify activation of p-JUN in response to the stiff matrix and promoting phenotypic shifts. The identified chromatin accessibility-dependent effect of matrix stiffness may be responsible for various fibrotic diseases and provide insight into |
HostingRepository | iProX |
AnnounceDate | 2025-04-19 |
AnnouncementXML | Submission_2025-04-21_21:30:44.076.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenxue Zhao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Astral |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-21 21:29:45 | ID requested | |
⏵ 1 | 2025-04-21 21:30:44 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: hepatic stellate cell |
Contact List
Yujie Sun | |
---|---|
contact affiliation | Peking University |
contact email | sun_yujie@pku.edu.cn |
lab head | |
Wenxue Zhao | |
contact affiliation | Peking University |
contact email | zhaowenxue@stu.pku.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |