PXD063160 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Roles of the phosphorylated SF3B1 in the activated spliceosome - experiment 1 |
| Description | SF3B1 is essential for assembly of B-complex spliceosome and activation of the Bact complex and undergoes dynamic phosphorylation and dephosphorylation during the splicing cycle. We have recently showed that the N-terminus of SF3B1 is phosphorylated during spliceosome activation by a little studied cyclin-dependent kinase 11 (CDK11) and remains hyperphosphorylated in catalytically active spliceosomes. However, little is known about the function of this N-terminal phosphorylation, about factors that “read” it, or about a spliceosome intermediate generated by CDK11 targeting. Here we demonstrate that CDK11 inhibition blocks spliceosome in a new intermediate during B to Bact transition. We now show that, phosphorylated SF3B1 N-terminus is crucial for binding of proteins during spliceosome activation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-24 |
| AnnouncementXML | Submission_2026-02-24_07:33:45.561.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063160 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | David Potesil |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-21 11:50:52 | ID requested | |
| ⏵ 1 | 2026-02-24 07:33:46 | announced | |
Publication List
Keyword List
| submitter keyword: SF3B1 phosphorylation, SNIP1,RNA splicing |
Contact List
| Zbynek Zdrahal |
|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
| contact email | zdrahal@sci.muni.cz |
| lab head | |
| David Potesil |
|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics
CEITEC-MU
Masaryk University
Brno, Czech Republic |
| contact email | david.potesil@ceitec.muni.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063160 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063160
- Label: PRIDE project
- Name: Roles of the phosphorylated SF3B1 in the activated spliceosome - experiment 1
