PXD063150

PXD063150 is an original dataset announced via ProteomeXchange.

Title	Anticodon Engineered Transfer RNA (tRNASUAG) Inhibits Hepatitis B Virus Replication by Promoting Degradation of the Core Protein
Description	Current antiviral drugs targeting hepatitis B virus (HBV) still face challenges in achieving functional cure for chronic hepatitis B. Therefore, it is necessary to explore new therapeutic approaches and combination therapy strategies. Anticodon engineered transfer RNA (ACE-tRNA) has been widely applied in the treatment of genetic diseases caused by nonsense mutations. However, its role in antiviral therapy has not been reported yet. In this study, ACE-tRNAs were designed targeting the highly conserved stop codon (UAG) of HBV core protein (HBc). All designed ACE-tRNAs can read through the stop codon of HBc. Among them, tRNASUAG promoted HBc phosphorylation by introducing a phosphorylatable serine into the C-terminal domain of HBc, thereby reducing HBc levels and inhibiting HBV replication by promoting the degradation of HBc through the ubiquitin-proteasome pathway. In addition, ACE-tRNA and CRISPR/Cas9 technologies can be effectively integrated through a tRNASUAG-gRNA tandem array and achieve the combined inhibition of HBV replication. This is the first study to use ACE-tRNA to promote HBc degradation by introducing an amino acid that can be post-translationally modified and subsequently inhibit HBV replication. In addition, this study presents a promising therapeutic strategy for promoting the clearance of HBV infection by integrating ACE-tRNA and CRISPR/Cas9 technologies.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:14:24.955.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xingwen Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-21 00:39:08	ID requested
⏵ 1	2025-12-07 16:14:25	announced

10.1002/advs.202503534;

Yang X, Sun H, Luo Z, Zhang Q, Song Y, Li J, Liu X, Wang J, ) Inhibits Hepatitis B Virus Replication by Promoting the Degradation of Core Protein. Adv Sci (Weinh), 12(45):e03534(2025) [pubmed]

submitter keyword: anticodon engineered transfer RNA

tRNASUAG

hepatitis B virus

core protein

ACE-tRNA-gRNA tandem array

Xingwen Yang
contact affiliation	Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center
contact email	2416397023@bjmu.edu.cn
lab head
Xingwen Yang
contact affiliation	Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center
contact email	2416397023@bjmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063150
     1. Label: PRIDE project
     2. Name: Anticodon Engineered Transfer RNA (tRNASUAG) Inhibits Hepatitis B Virus Replication by Promoting Degradation of the Core Protein