PXD063142

PXD063142 is an original dataset announced via ProteomeXchange.

Title	TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins Reveals Mechanisms of Microenvironment Adaptation
Description	Glioblastoma (GBM), the most aggressive primary brain tumor in adults, presents significant challenges due to its universal recurrence and limited survival rates. A key driver of GBM progression is the subpopulation of brain tumor-initiating cells (BTICs), which contribute to therapy resistance and interact with the tumor microenvironment, particularly the cellular components in the subventricular zone (SVZ). Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive molecules, such as proteins and RNAs, that can modulate the behavior of recipient cells. This study investigates the role of EVs in GBM's communication with non-cancer cells. We utilized the proximity-labeling system TurboID to achieve global and unbiased labeling of proteins within GBM-derived EVs. By inducing TurboID expression in primary-cultured human BTICs from GBM patients, we achieved efficient biotinylation of EV proteins without compromising vesicle integrity, and performed proteomic analysis of biotinylated proteins, which revealed a diverse cargo within BTIC-EVs. Our method marks the first implementation of TurboID for unbiased global labeling of EV protein cargo in primary cells. This approach facilitates the investigation of EV-mediated communication and potential therapeutic targets, contributing to the understanding of GBM's complex interactions with the brain's microenvironment, and identification of biomarkers for improved diagnosis and treatment response.
HostingRepository	PRIDE
AnnounceDate	2025-08-26
AnnouncementXML	Submission_2025-08-26_13:42:22.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TuKiet Lam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-20 22:40:56	ID requested
⏵ 1	2025-08-26 13:42:23	announced

Dataset with its publication pending

submitter keyword: brain tumor-initiating cells, Human brain tumor,extracellular vesicles, TurboID, glioblastoma multiforme, proximity protein labelling, subventricular zone, Cell specific proteomics, Cancer Stem Cells

TuKiet Lam
contact affiliation	Keck MS & Proteomics Resource Yale School of Medicine
contact email	tukiet.lam@yale.edu
lab head
TuKiet Lam
contact affiliation	Yale University
contact email	tukiet.lam@yale.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063142
     1. Label: PRIDE project
     2. Name: TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins Reveals Mechanisms of Microenvironment Adaptation