PXD063085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Whole Cell and Ubiquitinated Proteome of the Endoplasmic Reticulum (ER) Stress Response to Mild Hypothermic Culture Conditions in Chinese Hamsters Ovary Cells |
| Description | This study investigates the impact of mild hypothermic conditions (31°C) on the whole cell proteome and ubiquitinated proteome of Chinese hamster ovary (CHO) cells, with a specific focus on endoplasmic reticulum (ER) proteins and ER stress. Using high-resolution mass spectrometry, we conducted a comprehensive proteomic and ubiquitinated proteomic analysis to quantify changes in protein abundance and ubiquitinated peptides under mild hypothermia. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-21_23:39:46.330.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063085 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | David Ryan |
| SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: NEWT:10029; |
| ModificationList | ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-17 18:23:20 | ID requested | |
| ⏵ 1 | 2025-12-21 23:39:47 | announced | |
Publication List
| 10.6019/PXD063085; |
| Ryan D, Sideri CK, Henry M, Karuppuchamy S, Efeoglu E, Meleady P, Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions. Biotechnol Bioeng, 123(1):5-25(2026) [pubmed] |
| 10.1002/bit.70081; |
Keyword List
| submitter keyword: Label free, Cell Culture,CHO, Ubiquitin, LC-MS/MS |
Contact List
| Paula Meleady |
|---|
| contact affiliation | 1 Life Sciences Institute, Dublin City University, Glasnevin, Dublin 9, Ireland. 2 School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. 3 SSPC, The Science Foundation Ireland Research Centre for Pharmaceuticals, Limerick, Ireland |
| contact email | Paula.Meleady@dcu.ie |
| lab head | |
| David Ryan |
|---|
| contact affiliation | School of Biotechnology, Dublin City University |
| contact email | david.ryan68@mail.dcu.ie |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD063085 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063085
- Label: PRIDE project
- Name: Whole Cell and Ubiquitinated Proteome of the Endoplasmic Reticulum (ER) Stress Response to Mild Hypothermic Culture Conditions in Chinese Hamsters Ovary Cells
