PXD063063

PXD063063 is an original dataset announced via ProteomeXchange.

Title	Taxonomic identification and sex determination of Bos and Bison teeth using low-invasive high-resolution mass spectrometry
Description	Differentiating between the genus Bos and Bison from fossil bones and teeth can be difficult and even impossible due to taphonomic deterioration, which can also muddle the identification of the sex of the animal. Both are key issues for palaeobiological and palaeoecological studies, as the two species are generally considered having quite similar spatial distributions but distinct ecological preferences, and likely similar social behaviours with males separated from female groups during most of the year. However, identifications are usually limited to “Bos/Bison” and “Sex indeterminate”, which severely limits interpretations. Here, we propose a low-invasive mass spectrometry method for simultaneous taxonomic and sex determination of Bos and Bison teeth, with successful application on a Middle Pleistocene large bovid teeth from Lazaret cave. The peptides and proteins present in the enamel were directly extracted at the surface of the teeth. The proteins were then digested with trypsin or chymotrypsin and analysed by LC-MS/MS. The results obtained show that the enamelin, COL1A3 and alpha-2-glycoprotein allow for taxonomic differentiation between Bos and Bison, while AMELX/Y sequences of the same samples allow for sex identification. Both were successfully performed on the 160-120 ky archaeological teeth, without affecting the specimen. This study highlights the potential of proteomics for simultaneous taxonomic and sex determination for others fossil samples, including rare or precious material, using low-invasive high-resolution mass spectrometry. It opens up unprecedent avenues for palaeobiological studies as well as cultural and natural heritage, and will widely participate to strengthen our knowledge of past animal and human communities. Such multi-proxy approach, shall be now developed for other mammalian species.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-16_07:22:58.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Bison bonasus; NCBI TaxID: 9902; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Bison bison bison; NCBI TaxID: 43346; scientific name: Bos primigenius; NCBI TaxID: 9909;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-17 04:31:31	ID requested
⏵ 1	2025-06-16 07:22:58	announced

10.1021/ACS.JPROTEOME.5C00219;

submitter keyword: Bison, Tooth, Bos,Palaeoproteomics, Large bovids, Enamel,Genus identification, Sexing

Bray Fabrice
contact affiliation	MSAP UAR 3290 CNRS bat C4 avenue paul langevin 59655 villeneuve d'ascq cité scientifique
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063063
     1. Label: PRIDE project
     2. Name: Taxonomic identification and sex determination of Bos and Bison teeth using low-invasive high-resolution mass spectrometry