PXD063052

PXD063052 is an original dataset announced via ProteomeXchange.

Title	Quantitative Glycoproteomics Reveals Altered site-specific N-glycosylation Regarding Impaired Function of Extracellular Vesicles in Spermatozoa and Seminal Plasma of Asthenozoospermic Patients
Description	We quantified 127 and 118 glycoproteins with different expressed N-glycosylation in the spermatozoa and seminal plasma of asthenozoospermic patients, with that more significant in the seminal plasma. GO analysis revealed these glycoproteins were highly enriched in extracellular vesicles (EVs) and many of them were pronounced EVs markers such as CD59, CD63 and Tetraspanins-8. Besides, we observed remarkable changes in N-glycosylations of Galectin-3 which is a glycoprotein important for fertilization located on sperm mainly transferred by EVs in seminal plasma. Furthermore, expression of some N-glycans in specific glycosites were strongly correlated to progressive motility.
HostingRepository	iProX
AnnounceDate	2025-04-17
AnnouncementXML	Submission_2025-04-17_00:25:19.417.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-17 00:24:50	ID requested
⏵ 1	2025-04-17 00:25:19	announced

Dataset with its publication pending

submitter keyword: Human Spermatozoa, Seminal plasma, Glycosylation, Glycoproteomics, Asthenozoospermia

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI