PXD063051 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | lung cancer sEV uptake by T cells |
Description | Despite advances in immunotherapy, non-small cell lung carcinoma (NSCLC)’s clinical success is limited, possibly due to substantial immunological alterations in advanced cancer patients. This study examines the immunomodulatory effects of sEVs derived from lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC) on T cells. Methods: SEVs were isolated from lung cancer cell lines and Jurkat-E6.1. SEV size and morphology were analysed by NTA and TEM, respectively, while western blotting confirmed sEV markers. SEV uptake was assessed, followed by resazurin assay, RNA isolation, quantification, cDNA preparation, RT-PCR, nano LC-MS, and bioinformatic analysis, before and after treating Jurkat-E6.1 cells with sEVs from A549 and SKMES1. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-02 |
AnnouncementXML | Submission_2025-06-01_16:19:41.590.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063051 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hafiza Padinharayil |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-04-16 23:52:33 | ID requested | |
⏵ 1 | 2025-06-01 16:19:42 | announced | |
Publication List
Padinharayil H, Varghese J, Varghese PR, Wilson CM, George A, Small Extracellular Vesicle (sEV) Uptake from Lung Adenocarcinoma and Squamous Cell Carcinoma Alters T-Cell Cytokine Expression and Modulates Protein Profiles in sEV Biogenesis. Proteomes, 13(2):(2025) [pubmed] |
10.3390/proteomes13020015; |
10.6019/PXD063051; |
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: lung cancer, exosome, uptake, cross-treatment |
Contact List
Alex George |
contact affiliation | Lab Head and Scientist D, Jubilee Centre for Medical Research, JMMC&RI, Kerala, India |
contact email | alexgeorge@jmmc.ac.in |
lab head | |
Hafiza Padinharayil |
contact affiliation | Jubilee Mission Medical College and Research Institute |
contact email | hafizaaficvd@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063051
- Label: PRIDE project
- Name: lung cancer sEV uptake by T cells