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PXD063051

PXD063051 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlelung cancer sEV uptake by T cells
DescriptionDespite advances in immunotherapy, non-small cell lung carcinoma (NSCLC)’s clinical success is limited, possibly due to substantial immunological alterations in advanced cancer patients. This study examines the immunomodulatory effects of sEVs derived from lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC) on T cells. Methods: SEVs were isolated from lung cancer cell lines and Jurkat-E6.1. SEV size and morphology were analysed by NTA and TEM, respectively, while western blotting confirmed sEV markers. SEV uptake was assessed, followed by resazurin assay, RNA isolation, quantification, cDNA preparation, RT-PCR, nano LC-MS, and bioinformatic analysis, before and after treating Jurkat-E6.1 cells with sEVs from A549 and SKMES1.
HostingRepositoryPRIDE
AnnounceDate2025-06-02
AnnouncementXMLSubmission_2025-06-01_16:19:41.590.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD063051
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHafiza Padinharayil
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-16 23:52:33ID requested
12025-06-01 16:19:42announced
Publication List
Padinharayil H, Varghese J, Varghese PR, Wilson CM, George A, Small Extracellular Vesicle (sEV) Uptake from Lung Adenocarcinoma and Squamous Cell Carcinoma Alters T-Cell Cytokine Expression and Modulates Protein Profiles in sEV Biogenesis. Proteomes, 13(2):(2025) [pubmed]
10.3390/proteomes13020015;
10.6019/PXD063051;
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
submitter keyword: lung cancer, exosome, uptake, cross-treatment
Contact List
Alex George
contact affiliationLab Head and Scientist D, Jubilee Centre for Medical Research, JMMC&RI, Kerala, India
contact emailalexgeorge@jmmc.ac.in
lab head
Hafiza Padinharayil
contact affiliationJubilee Mission Medical College and Research Institute
contact emailhafizaaficvd@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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