PXD063051

PXD063051 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	lung cancer sEV uptake by T cells
Description	Despite advances in immunotherapy, non-small cell lung carcinoma (NSCLC)’s clinical success is limited, possibly due to substantial immunological alterations in advanced cancer patients. This study examines the immunomodulatory effects of sEVs derived from lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC) on T cells. Methods: SEVs were isolated from lung cancer cell lines and Jurkat-E6.1. SEV size and morphology were analysed by NTA and TEM, respectively, while western blotting confirmed sEV markers. SEV uptake was assessed, followed by resazurin assay, RNA isolation, quantification, cDNA preparation, RT-PCR, nano LC-MS, and bioinformatic analysis, before and after treating Jurkat-E6.1 cells with sEVs from A549 and SKMES1.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-01_16:19:41.590.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063051
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hafiza Padinharayil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-16 23:52:33	ID requested
⏵ 1	2025-06-01 16:19:42	announced

Publication List

Padinharayil H, Varghese J, Varghese PR, Wilson CM, George A, Small Extracellular Vesicle (sEV) Uptake from Lung Adenocarcinoma and Squamous Cell Carcinoma Alters T-Cell Cytokine Expression and Modulates Protein Profiles in sEV Biogenesis. Proteomes, 13(2):(2025) [pubmed]
10.3390/proteomes13020015;
10.6019/PXD063051;

Padinharayil H, Varghese J, Varghese PR, Wilson CM, George A, Small Extracellular Vesicle (sEV) Uptake from Lung Adenocarcinoma and Squamous Cell Carcinoma Alters T-Cell Cytokine Expression and Modulates Protein Profiles in sEV Biogenesis. Proteomes, 13(2):(2025) [pubmed]

10.3390/proteomes13020015;

10.6019/PXD063051;

Keyword List

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
submitter keyword: lung cancer, exosome, uptake, cross-treatment

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: lung cancer, exosome, uptake, cross-treatment

Contact List

Alex George
contact affiliation	Lab Head and Scientist D, Jubilee Centre for Medical Research, JMMC&RI, Kerala, India
contact email	alexgeorge@jmmc.ac.in
lab head
Hafiza Padinharayil
contact affiliation	Jubilee Mission Medical College and Research Institute
contact email	hafizaaficvd@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD063051
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD063051

PRIDE project URI

Repository Record List

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