PXD063019

PXD063019 is an original dataset announced via ProteomeXchange.

Title	Lipids accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution
Description	Oleaginous yeasts are used commercially to produce oleochemicals and hold potential also for biodiesel production. In response to nitrogen or phosphorous limitation, oleaginous yeast accumulate triacylglycerol. Previous work has investigated potential mechanisms by which nutrient limitation induces lipid biosynthesis without verifying whether lipid biosynthesis flux is actually enhanced. Here we show, using 13C-glucose tracing, that in nitrogen or phosphorous limitation, lipid accumulation occurs without consistent increases in biosynthetic flux. Instead, the main driver of increased lipid pools is decreased growth-related dilution. This conclusion holds across two divergent oleaginous yeasts: Rhodotorula toruloides and Yarrowia lipolytica. Quantitative proteomics shows a substantial proteome reallocation in response to nitrogen and phosphorous limitation, with ribosomal proteins strongly down regulated, while lipid biosynthetic enzymes are preserved but not consistently upregulated in absolute quantity. Thus, nutrient limitation, rather than triggering greatly enhanced lipid synthesis, results in roughly sustained lipid enzyme levels and biosynthetic flux. Due to slower lipid dilution by cell division, this suffices to drive marked lipid accumulation.
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-27_11:58:12.776.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063019
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felix Keber
SpeciesList	scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Rhodotorula toruloides; NCBI TaxID: 5286;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-16 03:05:00	ID requested
⏵ 1	2025-08-27 11:58:13	announced

10.1016/J.YMBEN.2025.08.010;

10.6019/PXD063019;

submitter keyword: Yarrowia lipolytica,Rhodotorula toruloides, kinetic flux profiling, biodiesel, de novo lipogenesis, isotope tracing, oleaginous yeast, biofuel

Martin Helmut Wühr
contact affiliation	Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA Department of Molecular Biology, Princeton University, Princeton, NJ, USA
contact email	wuhr@princeton.edu
lab head
Felix Keber
contact affiliation	Princeton University
contact email	fkeber@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063019
     1. Label: PRIDE project
     2. Name: Lipids accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution