PXD063001

PXD063001 is an original dataset announced via ProteomeXchange.

Title	A developmental shift in venom toxin expression underpins a major prey switch in Australian Brown Snakes
Description	Many animals undergo morphological, physiological, and behavioral changes over the course of their life history. It has been proposed that some of these developmental changes evolve in concert with shifts in ecology, but there are relatively few clear examples, and identifying the underlying molecular mechanisms is challenging. Australian Brown Snakes (Pseudonaja spp.), one of Australia's most venomous snakes, may provide such an example, as previous work has shown that these species undergo a dietary shift from reptiles to mammals, accompanied by differences in venom activity. Here, we show that the venom composition of adult Brown Snakes is distinguished by the collective expression of four procoagulant toxin families, which are not expressed in juvenile life stages. We also find that the developmental timing of the onset of procoagulant toxin expression correlates with a functional shift in venom activity to one that disrupts mammalian prey’s blood-clotting system. We further demonstrate that these shifts in toxin expression and procoagulant activity, do not occur in the smallest Brown Snake species that has evolved to be a permanent lizard-eating specialist. Collectively, we show that the coordinated shift in toxin expression mediates a major venom change in Australian Brown Snakes, from a neurotoxin-dominated venom in juveniles, used for capturing small lizards, to a procoagulant venom in adults, used for subduing mammals. Our findings therefore provide a compelling example of how animals adapt to ecological challenges that they face at different life stages.
HostingRepository	PRIDE
AnnounceDate	2026-04-13
AnnouncementXML	Submission_2026-04-13_07:45:44.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cara Smith
SpeciesList	scientific name: Pseudonaja modesta; NCBI TaxID: NEWT:340912; scientific name: Pseudonaja textilis; NCBI TaxID: NEWT:8673; scientific name: Pseudonaja aspidorhyncha; NCBI TaxID: NEWT:2929635;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF; timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-15 15:27:19	ID requested
⏵ 1	2026-04-13 07:45:45	announced

Dataset with its publication pending

submitter keyword: None

Anthony Saviola
contact affiliation	University of Colorado School of Medicine, Department of Biochemistry & Molecular Genetics, Aurora, CO, USA
contact email	Anthony.Saviola@cuanschutz.edu
lab head
Cara Smith
contact affiliation	University of Colorado School of Medicine
contact email	cara.2.smith@cuanschutz.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063001

PRIDE project URI

• PRIDE
  1. PXD063001
     1. Label: PRIDE project
     2. Name: A developmental shift in venom toxin expression underpins a major prey switch in Australian Brown Snakes