PXD062994

PXD062994 is an original dataset announced via ProteomeXchange.

Title	Sulfur Starvation Induces an Fe-Replete Response and Attenuates Virulence Pathways in Pseudomonas aeruginosa PAO1
Description	Specific bacteria grown with alternative sulfur sources leads to metabolic reprogramming that includes the expression of sulfur-scavenging and antioxidant genes. To determine the detailed metabolic effect of sulfur starvation, P. aeruginosa PAO1 was grown in sulfur-free media alone and with the addition of sulfate and evaluated by label-free proteomics. Proteomics analyses identified 729 differentially expressed proteins from 2633 confidently identified proteins. Differentially expressed features were analyzed for pathway enrichment using STRING (proteomics),leveraging Gene Ontology and KEGG annotations. corresponding proteins involved in sulfur assimilation and metabolism, including those of the msu, ssu, and cys operons, showed an increased abundance during sulfur starvation. Sulfur starvation depressed Fe-uptake gene/pathway expression, including the pyoverdine (pvd), pyochelin (pch), and heme-uptake (phu and has) operons. Expression of genes from virulence pathways was also diminished, including quorum sensing, MDR efflux pumps, phenazine biosynthetic genes, and hydrolytic enzymes thought to be regulated by the ferric uptake regulator and PrrF sRNAs. In contrast, there was a significant increase in expression of proteins involved in Fe-storage, including bfrB, dps, and PA4880. Our study demonstrates that sulfur starvation leads to an Fe-replete response in P. aeruginosa PAO1 due to a disruption in Fe homeostasis and uncovered the suppression of critical virulence gene products during sulfur starvation.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:16:30.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tonya Zeczycki
SpeciesList	scientific name: Pseudomonas aeruginosa ATCC 700888; NCBI TaxID: NEWT:1163394; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-15 10:40:50	ID requested
⏵ 1	2025-11-09 16:16:31	announced

10.1186/s12866-025-04442-1;

Ugochukwu CG, Schwartz TS, Zeczycki TN, Goodwin DC, Ellis HR, Sulfur starvation induces an Fe-replete response and attenuates virulence pathways in Pseudomonas aeruginosa PAO1. BMC Microbiol, 25(1):708(2025) [pubmed]

submitter keyword: sulfur,Pseudomonas aeruginosa, oxidative stress, Fe-Replete, Virulence Pathways

Holly R. Ellis
contact affiliation	Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, NC, USA
contact email	ellish20@ecu.edu
lab head
Tonya Zeczycki
contact affiliation	Brody School of Medicine at ECU
contact email	zeczyckit@ecu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062994
     1. Label: PRIDE project
     2. Name: Sulfur Starvation Induces an Fe-Replete Response and Attenuates Virulence Pathways in Pseudomonas aeruginosa PAO1