PXD062965

PXD062965 is an original dataset announced via ProteomeXchange.

Title	Hippocampal proteome profiling of a mouse model with dysfunctional STAT1 signaling
Description	Signal transduction via the Signal Transducer and Activator of Transcription 1 (STAT1) pathway is indispensable for mediating the intracellular effects of interferon-α (IFN-α), interferon-γ (IFN-γ) and other cytokines in the brain and, thereby, crucial for antiviral and antibacterial responses during potential life-threatening CNS infections. However, the role of STAT1 signaling beyond the known IFN-α and IFN-γ effects in immediate antimicrobial defense is highly context-dependent, and studies in the existing literature using STAT1-targeted mouse models under normal physiological conditions remain scarce. Here, we characterized a STAT1 targeted-disruption mouse model (STAT1-/- mice) in the absence of infectious stimuli by quantitative proteome analysis. Soluble hippocampal protein extracts from five female animals per condition (WT, STAT1-/-) were processed with replicate injection, resulting in two technical replicates per biological replicate and, thus, in a total of 20 LC-MS runs to be compared. We utilized an ion mobility-enhanced version of a data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE). Label-free protein quantification revealed numerous differentially regulated proteins, many of which classified as products of IFN-regulated genes according to Interferome V2.01 (https://interferome.org/interferome/home.jspx), and/or of STAT1 target genes according to the ENCODE transcription factor targets dataset (https://maayanlab.cloud/Harmonizome/dataset/ENCODE+Transcription+Factor+Targets). By integration of transcriptome and proteome data, we detected significant alterations in hippocampal gene and protein expression profiles implicated in neuroinflammatory processes and neuroprotection.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_21:22:16.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-15 01:09:24	ID requested
⏵ 1	2025-07-13 21:22:17	announced

B, ü, schgens L, Hempel N, Methi A, Fischer A, Siering N, Piepkorn L, Jahn O, Meyer T, Wirths O, Behavioral assessment and gene expression changes in a mouse model with dysfunctional STAT1 signaling. Cell Commun Signal, 23(1):305(2025) [pubmed]

10.1186/s12964-025-02313-w;

submitter keyword: label-free protein quantification, interferon signaling, hippocampus, mass spectrometry, proteome analysis, transcriptome analysis,STAT signaling

Prof. Dr. Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062965
     1. Label: PRIDE project
     2. Name: Hippocampal proteome profiling of a mouse model with dysfunctional STAT1 signaling