PXD062913

PXD062913 is an original dataset announced via ProteomeXchange.

Title	Analysis of Nucleocytoplasmic Protein Separation in DPST-Crosslinked Human Neuroblastoma SY5Y Cells
Description	Following DPST crosslinking of human neuroblastoma cells, nuclear-cytoplasmic fractionation was performed using hypotonic lysis to obtain cytoplasmic and nuclear protein compartments. Through systematic analysis of crosslinking mass spectrometry data, we elucidated how subcellular compartmentalized microenvironments regulate spatial distribution of protein conformations and topological architecture of interaction networks. This study reveals the heterogeneous principles governing dynamic protein assembly between nuclear and cytoplasmic compartments in neuroblastoma cells.
HostingRepository	iProX
AnnounceDate	2025-04-14
AnnouncementXML	Submission_2025-08-10_21:18:17.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-14 00:34:39	ID requested
1	2025-04-14 00:37:01	announced
2	2025-08-06 20:40:07	announced	2025-08-07: Add info.
⏵ 3	2025-08-10 21:18:18	announced	2025-08-07: Add info. 2025-08-11: Add info

Zhou Y, Zhang B, Zhong B, Sun M, Zhang X, Zhang Y, Zhang L, Zhao Q, Gong Z, Liu M, Cross-Linker Performance: Insights into Structural Dynamics and Molecular Rigidity. JACS Au, 5(7):3649-3659(2025) [pubmed]

submitter keyword: crosslinking, nuclear, cytoplasmic

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Jing Chen
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	chenj1810@dicp.ac.cn
dataset submitter

iProX dataset URI