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PXD062910

PXD062910 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins present in LACTB solutions overexpressed and purified from E.coli by LC-MS/MS.
DescriptionNon-enzymatic D-isomerization of aspartic acid in proteins has been observed in lesions associated with age-related diseases, including cataracts and Alzheimer's disease. Since D-isomerization of Asp disrupts the physiological conformation of proteins, it has been postulated that D-isomerization of Asp in proteins is a key factor in the pathogenesis of age-related diseases. D-aspartyl endopeptidase (DAEP) activity, which cleaves proteins at the carboxy terminus of D-Asp and may induce degradation of abnormal proteins with D-isomerized Asp, has been observed in mitochondrial fractions of mammalian tissues. However, the specific proteins responsible for mammalian DAEP activity remain unknown. In this study, we identified serine beta-lactamase-like protein (LACTB) as a mammalian D-aspartyl endopeptidase. We overexpressed and purified LACTB from E. coli and found that wild-type LACTB exhibited DAEP activity, but the S164A mutant LACTB, which lacks the catalytic serine, did not. To confirm that there are no other proteins that could explain the difference in DAEP activity, we systematically identified proteins present in the purified solution of LACTB.
HostingRepositoryjPOST
AnnounceDate2025-04-19
AnnouncementXMLSubmission_2025-04-18_16:31:56.182.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGenta Ito
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListL-cysteine methyl disulfide; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-13 22:48:51ID requested
12025-04-18 16:31:56announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LACTB, D-aspartyl endopeptidase
Contact List
Genta Ito
lab head
Genta Ito
contact affiliationTeikyo University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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