PXD062910

PXD062910 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of proteins present in LACTB solutions overexpressed and purified from E.coli by LC-MS/MS.
Description	Non-enzymatic D-isomerization of aspartic acid in proteins has been observed in lesions associated with age-related diseases, including cataracts and Alzheimer's disease. Since D-isomerization of Asp disrupts the physiological conformation of proteins, it has been postulated that D-isomerization of Asp in proteins is a key factor in the pathogenesis of age-related diseases. D-aspartyl endopeptidase (DAEP) activity, which cleaves proteins at the carboxy terminus of D-Asp and may induce degradation of abnormal proteins with D-isomerized Asp, has been observed in mitochondrial fractions of mammalian tissues. However, the specific proteins responsible for mammalian DAEP activity remain unknown. In this study, we identified serine beta-lactamase-like protein (LACTB) as a mammalian D-aspartyl endopeptidase. We overexpressed and purified LACTB from E. coli and found that wild-type LACTB exhibited DAEP activity, but the S164A mutant LACTB, which lacks the catalytic serine, did not. To confirm that there are no other proteins that could explain the difference in DAEP activity, we systematically identified proteins present in the purified solution of LACTB.
HostingRepository	jPOST
AnnounceDate	2025-04-19
AnnouncementXML	Submission_2025-07-21_16:59:17.338.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Genta Ito
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	L-cysteine methyl disulfide; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-13 22:48:51	ID requested
1	2025-04-18 16:31:56	announced
⏵ 2	2025-07-21 16:59:18	announced	2025-07-22: Updated PubMed.

Publication List

Ito G, Utsunomiya-Tate N, Discovery and biochemical characterization of the D-aspartyl endopeptidase activity of the serine protease LACTB. J Biol Chem, 301(6):108549(2025) [pubmed]

Ito G, Utsunomiya-Tate N, Discovery and biochemical characterization of the D-aspartyl endopeptidase activity of the serine protease LACTB. J Biol Chem, 301(6):108549(2025) [pubmed]

Keyword List

submitter keyword: LACTB, D-aspartyl endopeptidase

submitter keyword: LACTB, D-aspartyl endopeptidase

Contact List

Genta Ito
lab head
Genta Ito
contact affiliation	Teikyo University
dataset submitter

Full Dataset Link List

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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003762/