PXD062910
PXD062910 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of proteins present in LACTB solutions overexpressed and purified from E.coli by LC-MS/MS. |
Description | Non-enzymatic D-isomerization of aspartic acid in proteins has been observed in lesions associated with age-related diseases, including cataracts and Alzheimer's disease. Since D-isomerization of Asp disrupts the physiological conformation of proteins, it has been postulated that D-isomerization of Asp in proteins is a key factor in the pathogenesis of age-related diseases. D-aspartyl endopeptidase (DAEP) activity, which cleaves proteins at the carboxy terminus of D-Asp and may induce degradation of abnormal proteins with D-isomerized Asp, has been observed in mitochondrial fractions of mammalian tissues. However, the specific proteins responsible for mammalian DAEP activity remain unknown. In this study, we identified serine beta-lactamase-like protein (LACTB) as a mammalian D-aspartyl endopeptidase. We overexpressed and purified LACTB from E. coli and found that wild-type LACTB exhibited DAEP activity, but the S164A mutant LACTB, which lacks the catalytic serine, did not. To confirm that there are no other proteins that could explain the difference in DAEP activity, we systematically identified proteins present in the purified solution of LACTB. |
HostingRepository | jPOST |
AnnounceDate | 2025-04-19 |
AnnouncementXML | Submission_2025-04-18_16:31:56.182.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Genta Ito |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | L-cysteine methyl disulfide; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-13 22:48:51 | ID requested | |
⏵ 1 | 2025-04-18 16:31:56 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LACTB, D-aspartyl endopeptidase |
Contact List
Genta Ito | |
---|---|
lab head | |
Genta Ito | |
contact affiliation | Teikyo University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003762/ |