PXD062886

PXD062886 is an original dataset announced via ProteomeXchange.

Title	The accessory type III secretion system effectors shape inflammatory infection outcome
Description	Injection of effectors via a type III secretion system (T3SS) is an infection strategy shared by many Gram-negative bacterial pathogens. While individual T3SS effectors are well characterized, their network-level organization and the distinction between core and accessory effectors remain incompletely understood. Here, we systematically dissect the T3SS effector network from Citrobacter rodentium (CR), identifying a subset of 12 accessory effectors that, while dispensable for colonization, significantly alter infection outcomes. A strain lacking these effectors (CRM12) remained virulent in susceptible hosts yet resulted in reduced epithelial barrier damage, inflammation and immune cell infiltration in resistant mouse hosts. Deep proteomic analysis specifically targeting CR-attached colonic epithelial cells revealed that, despite lacking 39% of its effector repertoire, infection with CRM12 results in similar changes to global protein expression as seen in mice infected with the wild-type strain, though key regulators of barrier disruption were differentially expressed. Using a host model with impaired barrier repair, we confirmed that accessory effectors shape infection dynamics without significantly impacting virulence. This study thus refines the concept of core and accessory effectors, providing a basis for further studies into effector-driven host adaptation.
HostingRepository	PRIDE
AnnounceDate	2025-04-16
AnnouncementXML	Submission_2025-04-16_05:10:06.429.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Graeme Benstead-Hume
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-13 06:47:13	ID requested
⏵ 1	2025-04-16 05:10:06	announced

Dataset with its publication pending

submitter keyword: type III secretion system

Jyoti Choudhary
contact affiliation	Functional Proteomics, ICR
contact email	jyoti.choudhary@icr.ac.uk
lab head
Graeme Benstead-Hume
contact affiliation	ICR
contact email	ghume@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062886
     1. Label: PRIDE project
     2. Name: The accessory type III secretion system effectors shape inflammatory infection outcome