PXD062877

PXD062877 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of innate immune responses in Polg D257A macrophages challenged with Pseudomonas aeruginosa
Description	Mitochondrial diseases (MtD) represent a significant public health challenge due to their heterogenous clinical presentation, often severe and progressive symptoms, and lack of effective therapies. Environmental exposures, such bacterial and viral infection, can further compromise mitochondrial function and exacerbate the progression of MtD. Infections in MtD patients more frequently progress to sepsis, pneumonia, and other detrimental inflammatory endpoints. However, the underlying immune alterations that enhance immunopathology in MtD remain unclear, constituting a key gap in knowledge that complicates treatment and increases mortality in this vulnerable population. This data set utilizes an in vitro assay using Pseudomonas aeruginosa (PA) infection of bone marrow derived macrophages (BMDMs) to clarify the molecular and cellular basis for innate immune hyperactivity in a mouse model of polymerase gamma (Polg)-related MtD. This dataset reports the proteome signatures of WT and Polg D257A mutant BMDMs at rest and 2-8 hours post infection with PA.
HostingRepository	PRIDE
AnnounceDate	2025-04-14
AnnouncementXML	Submission_2025-04-14_10:39:35.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip West
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-12 10:23:29	ID requested
⏵ 1	2025-04-14 10:39:37	announced

10.1101/2024.05.11.593693;

VanPortfliet JJ, Lei Y, Ramanathan M, Martinez CG, Wong J, Stodola TJ, Hoffmann BR, Pflug K, Sitcheran R, Kneeland SC, Murray SA, McGuire PJ, Cannon CL, West AP, Caspase-11 drives macrophage hyperinflammation in models of Polg-related mitochondrial disease. bioRxiv, ():(2025) [pubmed]

submitter keyword: innate immunity, Polg,Mouse, bone marrow macrophage, mitochondrial dysfunction, Pseudomonas aeruginosa

Phillip West
contact affiliation	The Jackson Laboratory, Bar Harbor, ME, USA
contact email	phillip.west@jax.org
lab head
Phillip West
contact affiliation	The Jackson Laboratory
contact email	phillip.west@jax.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD062877

PRIDE project URI

• PRIDE
  1. PXD062877
     1. Label: PRIDE project
     2. Name: Proteomic analysis of innate immune responses in Polg D257A macrophages challenged with Pseudomonas aeruginosa