PXD062866

PXD062866 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of thermogenic adipocyte-derived extracellular vesicles in mice
Description	Thermogenic brown adipocytes (BAs) are not only an energy sink but also regulate systemic metabolic health through secreted factors and extracellular vehicles (EVs). Here we investigate BA-EVs under both control and cold stimulation. We first established and validated transgenic mouse models expressing human CD63 (hCD63)-GFP for visualization and isolation of EVs exclusively from adipocytes and BAs. We then used super-resolution microscopy to compare the subcellular localization and maturation of EVs in adipocytes grown in culture or directly isolated from mice, revealing distinct features. We further used dynabeads to enrich BA-EVs and employed mass spectrometry to define the EV-proteomes. We discovered that cold-stimulation elevated contents of mitochondrial and lysosomal proteins in BA-EVs. We validated the presence and activity of creatine kinase B (CKB) in BA-EVs. We finally showed that BA-EVs promote thermogenic gene expression in white adipocytes, but cold-stimulation surprisingly blunted EV’s effect in up-regulating Prdm16 expression. These data establish a novel system for analyzing EV contents in vivo and provide insight into how EV proteomes dynamically adapt to non-shivering thermogenesis.
HostingRepository	jPOST
AnnounceDate	2025-04-12
AnnouncementXML	Submission_2025-04-11_21:21:44.216.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Kai Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-11 15:06:28	ID requested
⏵ 1	2025-04-11 21:21:44	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Extracellular vesicles, Thermogenic adipocyte, Creatine kinase B

submitter keyword: Extracellular vesicles, Thermogenic adipocyte, Creatine kinase B

Contact List

Shihuan Kuang
lab head
Yi-Kai Liu
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003757/