PXD062862

PXD062862 is an original dataset announced via ProteomeXchange.

Title	Proximity labelling of the post-Golgi AP complexes in Plasmodium falciparum
Description	Adaptor protein (AP) complexes are pivotal components of the post-Golgi trafficking and endocytosis process and are involved in the selection, package and sorting of cargo into vesicles to different destination compartments in eukaryotic cells. The main objective of this project was to investigate the proxiome of the post-Golgi adaptors AP-1, AP-3 and AP-4 in the human malaria parasite Plasmodium falciparum, using the DiQ-BioID approach. To this end, malaria parasites were genetically modified via selection linked integration (SLI) to express endogenously 2xFKBR-GFP-2xFKBP-tagged versions of the μ subunits of AP-1, AP-3 and AP-4 complexes, followed by transfections with plasmids to episomally express miniTurbo-FRB-mCherry (N-terminal) and mCherry-FRB-miniTurbo (C-terminal). The conditional recruitment of the miniTurbo to AP foci (trans-Golgi localisation) was induced by addition of rapalog. Consequently, a mass spectrometry analysis of biotinylated proteins resulted in a list of high-confidence hits for each adaptor, which identified potential interactors and compartment neighbours. The resulting data provides an unconventional insight into the vesicle trafficking and cargo sorting machinery in malaria parasites.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_12:49:19.887.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gala Ramon-Zamorano
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 120

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-11 11:19:50	ID requested
⏵ 1	2025-09-17 12:49:20	announced

10.1083/JCB.202504062;

submitter keyword: adaptor protein (AP) complexes,Malaria, vesicular trafficking., miniTurbo, proximity labelling, Plasmodium falciparum, SLI, DiQ-BioID

Tobias Spielmann
contact affiliation	Bernhard Nocht Institute for Tropical Medicine
contact email	spielmann@bnitm.de
lab head
Gala Ramon-Zamorano
contact affiliation	Bernhard-Nocht-Institut für Tropenmedizin
contact email	gala.ramon-zamorano@bnitm.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062862

PRIDE project URI

• PRIDE
  1. PXD062862
     1. Label: PRIDE project
     2. Name: Proximity labelling of the post-Golgi AP complexes in Plasmodium falciparum