PXD062857 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BatR: A novel regulator of sub-inhibitory antibiotic tolerance in Pseudomonas aeruginosa biofilms |
| Description | Pseudomonas aeruginosa is a multidrug-resistant opportunistic pathogen, with chronic infections often associated with biofilms that enhance antibiotic resistance. This study investigates the uncharacterized gene PA3049, which is upregulated under biofilm conditions, to determine its role in infection, biofilm formation, and antimicrobial tolerance. o Using bioinformatics, infection models, and molecular microbiology, we determined that PA3049 contributed to biofilm establishment both in vitro and in high-validity infection models. We also identified its role in bacterial survival under sub-inhibitory concentrations of antibiotics and its impact on pyocyanin production. Proteomic analysis revealed that PA3049 upregulates the R2-type pyocin cluster, which drives explosive cell lysis and extracellular DNA (eDNA) release during early stages of P. aeruginosa biofilm development. Additionally, PA3049 interacts with PA0486, an uncharacterized Ser/Thr protein kinase implicated in pyocyanin production and bacterial killing, suggesting a putative in vivo mechanism of action. o Renamed as the Biofilm antibiotic tolerance Regulator (BatR), PA3049 emerges as a key player in P. aeruginosa biofilm maintenance and resistance. These findings provide new insights into bacterial biofilm dynamics and highlight two previously uncharacterized genes with potential implications for combating multidrug-resistant infections |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-26 |
| AnnouncementXML | Submission_2026-06-26_08:54:46.324.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD062857 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Gerhard Saalbach |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: NEWT:208964; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-11 09:04:31 | ID requested | |
| ⏵ 1 | 2026-06-26 08:54:46 | announced | |
Publication List
Keyword List
| submitter keyword: Pseudomonas aeruginosa |
| biofilms |
| antibiotic resistance |
| proteomics |
Contact List
| Jacob Malone |
|---|
| contact affiliation | Group Leader Dept Molecular Microbiology John Innes Centre Norwich, UK |
| contact email | jacob.malone@jic.ac.uk |
| lab head | |
| Gerhard Saalbach |
|---|
| contact affiliation | Biological Chemistry |
| contact email | gerhard.saalbach@jic.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062857
- Label: PRIDE project
- Name: BatR: A novel regulator of sub-inhibitory antibiotic tolerance in Pseudomonas aeruginosa biofilms
