PXD062840

PXD062840 is an original dataset announced via ProteomeXchange.

Title	Unraveling the proteomic landscape of red-fleshed apples ‎to identify regulators of anthocyanin accumulation
Description	Anthocyanins are colorful plant pigments with antioxidant properties, and a diet rich in these ‎flavonoids bears health benefits. Therefore, a strong anthocyanin accumulation in edible plant ‎parts is of significant interest, and in Malus domestica, the domesticated apple, certain red-fleshed ‎apple varieties exhibit this trait. Enhanced anthocyanin accumulation in the flesh of apple fruits is ‎attributed to the hyperactivation of the MYB transcription factor MdMYB10, which act as a key ‎regulators of anthocyanin biosynthesis by inducing the expression of multiple biosynthetic genes. ‎While several studies have explored the underlying genetic mutations and resulting transcriptome ‎changes, there is a lack of research on proteome alterations that cause the red-fleshed apple ‎phenotype. To address this gap, a mass spectrometry-based proteomics approach was employed. ‎Comparative proteomics identified differentially abundant proteins in young and mature fruits of the ‎red-fleshed ‘Bay13645’ variety compared to the white-fleshed ‘Royal Gala’. Whereas several MYB ‎transcription factors were enriched during early fruit development, they were no longer among the ‎hyper-abundant proteins in ripe fruits of the red-fleshed genotype. In contrast, anthocyanin ‎biosynthetic enzymes were enriched more strongly in ripe fruits of the red-fleshed cultivar, ‎indicating developmental stage-specific differences in the control of the pigmentation process. The ‎proteomic approach also identified novel regulatory factors and enzymes that may contribute to the ‎red-fleshed apple phenotype, including a BAHD acyltransferase, Mal d proteins, and transcription ‎factors of diverse families, and their potential relevance for the exhibition of this trait is discussed.‎
HostingRepository	PRIDE
AnnounceDate	2025-06-06
AnnouncementXML	Submission_2025-06-06_02:17:28.571.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062840
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qussai Abbas
SpeciesList	scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-11 00:47:53	ID requested
⏵ 1	2025-06-06 02:17:29	announced

10.6019/PXD062840;

10.1016/J.JPROT.2025.105470;

submitter keyword: Mal d proteins, Malus domestica,Acyltransferase, MYB transcription factors, fruits

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, ‎Freising, ‎Germany
contact email	kuster@tum.de
lab head
Qussai Abbas
contact affiliation	Technical University of Munich
contact email	qussai.abbas@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062840
     1. Label: PRIDE project
     2. Name: Unraveling the proteomic landscape of red-fleshed apples ‎to identify regulators of anthocyanin accumulation