PXD062828
PXD062828 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neutralization mechanism of a HipA-like toxin targeting isoleucyl-tRNA synthetase |
Description | HipAB is a type II toxin-antitoxin (TA) system that targets and inactivates specific aminoacyl-tRNA synthetases in response to various stresses. While the molecular targets and neutralization mechanisms of several HipAB-like TA systems have been well characterized, their structural and functional diversity remains less understood. In this study, we investigated a HipAB-like module (HipABpf) from Pseudomonas fluorescens, where the HipBpf antitoxin features a long, disordered C-terminal region in the absence of HipApf. Using a combination of X-ray crystallography, AlphaFold modeling and mutagenesis assays, we show that upon binding to HipApf, part of this C-terminal region forms two α-helices that are essential for both the interaction with and neutralization of the HipApf toxin. Importantly, HipBpf binding blocks the ATP binding sites of HipApf, potentially by inducing a conformational change in the HipApf N1 subdomain via its C-terminal α6 helix. We also discovered that HipApf phosphorylates the isoleucyl-tRNA synthetase at Ser604, strongly inhibiting its aminoacylation activity. Finally, we demonstrated that the HipABpf system plays a role in coping with oxidative stress and carbon starvation. Taken together, our findings reveal the critical role of the HipBpf C-terminal region in toxin binding and neutralization, while also highlighting the evolutionarily divergent substrate preferences of HipA-like toxins. |
HostingRepository | iProX |
AnnounceDate | 2025-04-11 |
AnnouncementXML | Submission_2025-04-10_20:41:04.076.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Siping Zhang |
SpeciesList | scientific name: Escherichia coli K-12; NCBI TaxID: 83333; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-10 20:40:52 | ID requested | |
⏵ 1 | 2025-04-10 20:41:04 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HipAB system,Pseudomonas fluorescens,Phosphorylation,Neutralization mechanism |
Contact List
Yongxing He | |
---|---|
contact affiliation | Lanzhou University |
contact email | heyx@lzu.edu.cn |
lab head | |
Siping Zhang | |
contact affiliation | Lanzhou University |
contact email | zhangsp2015@lzu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |