PXD062827

PXD062827 is an original dataset announced via ProteomeXchange.

Title	Nanoparticle-based Antigen Favors High Level of Humoral Immune Responses and Increases Antigenicity of Highly Glycosylated Protein
Description	Nanoparticles (NPs) have shown great potential as advanced vaccines and immunotherapy platforms due to their size and polyvalent epitopes. However, the immune responses elicited by glycosylated antigens conjugated to NPs with variable antigen densities remain poorly understood. In this study, we analyzed the glycosylation profile of the CD2v ectodomain (ED) of African swine fever virus, identifying 14 potential N-glycosylation sites (PNGS) among 192 residues, which markedly reduced its antigenicity. To enhance the antigenicity of CD2v, we designed three protein-based NPs derived from Ferritin, porcine circovirus type 2 (PCV2) capsid protein, and AP205 coat protein. Antigens were conjugated to the NP surfaces using the SpyTag/SpyCatcher system. Immunoassays demonstrated that these NPs rapidly induced higher levels of antigen-specific IgG compared to unconjugated antigens, with the effect being most pronounced for the highly glycosylated CD2v. All NPs significantly promoted IgG2a production, indicating a Th1-biased immune response. Furthermore, PCV2 NP reached the popliteal lymph node (PLN) within 5 min post-injection and persisted for 21 days, extensively interacting with B cells. These findings provide the potential of tailoring NP design to enhance antigenicity, providing a foundation for developing glycosylated antigen-targeted vaccines and immunotherapies.
HostingRepository	iProX
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-10_20:39:28.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yawen Zou
SpeciesList	scientific name: African swine fever virus; NCBI TaxID: 10497;
ModificationList	glycosylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 20:39:08	ID requested
⏵ 1	2025-04-10 20:39:28	announced

Dataset with its publication pending

submitter keyword: Nanoparticles, Glycoprotein, Popliteal lymph node

Yi Yang
contact affiliation	Hunan Agricultural University
contact email	yiyang@hunau.edu.cn
lab head
Yawen Zou
contact affiliation	Hunan Agricultural University
contact email	yawenzou@stu.hunau.edu.cn
dataset submitter

iProX dataset URI