PXD062818

PXD062818 is an original dataset announced via ProteomeXchange.

Title	Nigericin-Triggered Phosphodynamics in Inflammasome Formation and Pyroptosis
Description	Innate immune signaling relies on phosphorylation cascades for effective responses. While TLR4 stimulation has been well-studied, research on signaling after inflammasome triggers like nigericin is limited. We performed time-resolved phosphoproteomic profiling to explore kinase signaling downstream of nigericin and discovered rapid changes in phosphorylation, particularly involving immune and MAPK pathways. Notably, we identified fluxes in PKC signaling and calcium-responsive proteins, along with phospho-modified metabolic pathways, indicating a role in immunometabolic regulation. Additionally, significant changes in DNA reorganization and damage response proteins linked to pyknosis were observed. Temporal clustering of phospho-dynamics revealed new shifts in signaling cascades after nigericin treatment, underscoring changes in cellular behavior related to inflammatory events
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-27_14:51:21.221.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 11:33:46	ID requested
⏵ 1	2025-08-27 14:51:21	announced

10.1002/PMIC.70030;

submitter keyword: innate immunity, Signalling Pathways,Phosphoproteomics, Pyroptosis, Inflammasomes

Aleksandra Nita-Lazar
contact affiliation	NIAID, NATIONAL INSTITUTES OF HEALTH
contact email	nitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD062818

PRIDE project URI

• PRIDE
  1. PXD062818
     1. Label: PRIDE project
     2. Name: Nigericin-Triggered Phosphodynamics in Inflammasome Formation and Pyroptosis