PXD062807

PXD062807 is an original dataset announced via ProteomeXchange.

Title	Chaperone-mediated autophagy controls brown adipose tissue 2 thermogenic activity
Description	The activity of brown fat, a tissue mediating non-shivering thermogenesis, is associated with protection against obesity, diabetes, and cardiovascular disease. In response to thermogenic stimuli, brown adipose tissue (BAT) increases its activity through extensive cellular and tissue plasticity. While macroautophagy is typically inhibited during BAT thermogenic activation, we found that chaperone-mediated autophagy (CMA), a selective type of autophagy, is instead induced. CMA induction enhances both basal and cAMP-stimulated activity of brown adipocytes, as evidenced by increased expression of thermogenesis-related genes, enhanced release of brown adipokines, elevated cellular oxidative activity, and increased lipolysis. In aging, when BAT activity declines, LAMP2A—the limiting CMA component—is downregulated in BAT. However, pharmacological activation of CMA restores BAT activity in aged mice. To investigate the consequences of this CMA failure in BAT, we generated brown adipocytes knock down for LAMP2A and using comparative proteomics identified that CMA regulates levels of proteins involved in BAT thermogenic and metabolic activity. We demonstrate that selective CMA blockade in interscapular BAT in mice leads to reduced energy expenditure, increased triglyceridemia, lower expression of thermogenic markers and BAT-secretory functions, and to accumulation of thermogenesis repressors upon thermogenic activation. Overall, these findings support the essential role of CMA in BAT function and adaptation to thermogenic activation. CMA facilitates the timely degradation of thermogenic repressors, thereby promoting adaptive enhancement of thermogenic activity.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_06:56:55.786.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tania Paloma Quesada-López
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 05:26:15	ID requested
⏵ 1	2025-09-30 06:56:56	announced

Dataset with its publication pending

submitter keyword: proteomics, brown adipose tissue, thermogenesis,Autophagy

Joan Villarroya Terrade
contact affiliation	Department of Biochemistry and Molecular Biomedicine and Institute of Biomedicine 10 (IBUB), University of Barcelona, Barcelona, Spain CIBER “Fisiopatología de la Obesidad y Nutrición”, Instituto de Salud Carlos III, Madrid, Spain
contact email	jvillarroya@ub.edu
lab head
Tania Paloma Quesada-López
contact affiliation	University of Barcelona
contact email	tquesada@ub.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062807

PRIDE project URI

• PRIDE
  1. PXD062807
     1. Label: PRIDE project
     2. Name: Chaperone-mediated autophagy controls brown adipose tissue 2 thermogenic activity