PXD062804

PXD062804 is an original dataset announced via ProteomeXchange.

Title	Engineering yeast peroxisome assembly enables increased production of acetyl-CoA and derived 5-deoxyflavonoids
Description	Acetyl-CoA serves as foundational precursor and energy source for various biosynthesis pathways. The insufficient supply of acetyl-CoA in cytosol is usually a bottleneck for exogenous chemical synthesis in engineered microbes such as yeast. Exportation of acetyl-CoA from peroxisomes, an exclusive organelle for fatty acids β-oxidation, may be an effective way to solve this problem. In this study, we developed a peroxin (PEX) engineering strategy to modulate peroxisome assembly in S. cerevisiae. Then, robust peroxisomes were constructed with improved acetyl-CoA supply by up to 98%, which further led to the increased liquiritigenin titer (1102.41 mg/L), the highest reported value so far. We also demonstrated that the PEX–mediated peroxisome engineering strategy can be extended across yeast species. Hybrid peroxisomes with tailored function were constructed in S. cerevisiae by transplanting selected PEXs from Y. lipolytica. Our study provides novel mechanistic insights into the “PEXs–peroxisome assembly–acetyl-CoA synthesis” relationship.
HostingRepository	iProX
AnnounceDate	2025-04-10
AnnouncementXML	Submission_2025-04-10_01:18:50.072.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongxing Li
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 01:16:03	ID requested
⏵ 1	2025-04-10 01:18:50	announced

Dataset with its publication pending

submitter keyword: LC-MSMS

Xudong Feng
contact affiliation	Beijing Institute of Technology
contact email	xd.feng@bit.edu.cn
lab head
Yongxing Li
contact affiliation	Beijing Institute of Technology
contact email	liyongxing950827@163.com
dataset submitter

iProX dataset URI