PXD062783

PXD062783 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles released from cortical neurons influence spontaneous activity of recipient neurons.
Description	Extracellular vesicles (EVs) are membranous structures that cells release into the extracellular space. EVs carry various molecules such as proteins, lipids, and nucleic acids, and serve as specialized transporters to influence other cells. In the central nervous system, EVs have been linked to many important processes, including intercellular communication, but molecular details of their physiological functions are not fully understood. Our study aimed to investigate how EVs are released by neuronal cells, and how they affect the neuronal activity of other recipient neurons. We show that mature primary cortical neurons release EVs from both their soma and dendrites. EVs released from neurons closely resemble non-neuronal EVs regarding size and marker proteins and proteomic analyses showed that neuronally released EVs contain proteins typically acting in pre- and post-synaptic compartments. Interestingly, our analysis revealed that EVs alter spontaneous activity in target neurons by increasing the amplitude of postsynaptic potentials. In summary, our findings elaborates on the role of EVs in synaptic activity modulation in neurons mediated by glutamate receptors.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-21_16:13:50.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bente Siebels
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-09 09:26:19	ID requested
⏵ 1	2025-09-21 16:13:51	announced

Lombino FL, Shafiq M, Matamoros-Angles A, Schwarz JR, Gromova KV, Stajano D, Siebels B, Bergmann L, Magnus T, Schweizer M, Ricklefs FL, Schl, ü, ter H, Hill AF, Kneussel M, Glatzel M, Extracellular Vesicles Released From Cortical Neurons Influence Spontaneous Activity of Recipient Neurons. J Neurochem, 169(9):e70231(2025) [pubmed]

10.1111/jnc.70231;

submitter keyword: hippocampus, synaptic activity, NMDA receptor,Extracellular vesicles, cortex

Prof. Dr. med. Markus Glatzel
contact affiliation	Institute for Neuropathology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246, Hamburg, Germany
contact email	m.glatzel@uke.de
lab head
Bente Siebels
contact affiliation	Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	b.siebels@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062783

PRIDE project URI

• PRIDE
  1. PXD062783
     1. Label: PRIDE project
     2. Name: Extracellular vesicles released from cortical neurons influence spontaneous activity of recipient neurons.