PXD062742 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Dual effect of Staphylococcus epidermidis DnaK on Staphylococcus aureus biofilm proteostasis and regulation |
| Description | Staphylococcus aureus and Staphylococcus epidermidis, two Gram-positive bacteria commonly found in the human skin microbiota, form biofilms that contribute to skin dysbiosis and play a key role in conditions like acne and atopic dermatitis. The Calcitonin Gene-Related Peptide (CGRP) is a human peptide involved in skin inflammation. We previously showed that CGRP enhances the virulence of S. epidermidis MFP04 and that the DnaK chaperone protein is significantly overexpressed in the secretome of CGRP-activated virulent S. epidermidis. In this study, we explored a potential new role of S. epidermidis DnaK in biofilm formation in both S. aureus and S. epidermidis. We showed that recombinant S. epidermidis DnaK differentially affects biofilm formation, whether in two skin commensal staphylococcal strains (S. aureus MFP03 and S. epidermidis MFP04) or in a clinical S. aureus strain (CIP 107093). In the clinical strain S. aureus CIP 107093, biofilm formation was most strongly inhibited. This inhibition involves both the Substrate-Binding Domain and the Nucleotide-Binding Domain of DnaK. Proteomic analysis revealed that DnaK alters the S. aureus biofilm proteome, stabilizing proteins involved in protein degradation like ClpP and ETA, while downregulating key regulatory proteins involved in biofilm development such as SaeS and WalK. These results indicate that S. epidermidis DnaK may contribute to the regulation of S. aureus biofilm formation, suggesting a cross-species regulatory role of DnaK within the skin microbiota. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-09 |
| AnnouncementXML | Submission_2026-03-08_19:58:12.140.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Eléonore Bouscasse |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-08 09:27:51 | ID requested | |
| ⏵ 1 | 2026-03-08 19:58:13 | announced | |
Publication List
| Kayser C, Druart K, Bouscasse E, Matondo M, Chane A, Hoh F, Groboillot A, Barbey C, Merieau A, Latour X, Soule P, M, ü, hle E, Norris V, Konto-Ghiorghi Y, CIP 107093. Front Microbiol, 16():1705130(2025) [pubmed] |
| 10.3389/fmicb.2025.1705130; |
Keyword List
| submitter keyword: DnaK |
| biofilm |
| Staphylococcus epidermidis |
| Staphylococcus aureus |
| skin microbiota |
Contact List
| Yoan Konto-Ghiorghi |
|---|
| contact affiliation | Research Unit Bacterial Communication and Anti-infectious Strategies (CBSA, UR4312), University of Rouen Normandie, 27000 Evreux, France |
| contact email | Yoan.Konto-Ghiorghi@univ-rouen.fr |
| lab head | |
| Eléonore Bouscasse |
|---|
| contact affiliation | Institut Pasteur |
| contact email | eleonore.bouscasse@pasteur.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD062742 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062742
- Label: PRIDE project
- Name: Dual effect of Staphylococcus epidermidis DnaK on Staphylococcus aureus biofilm proteostasis and regulation
