PXD062737

PXD062737 is an original dataset announced via ProteomeXchange.

Title	Comparative LC-MSMS Analysis of Extracellular Vesicles Extracted from Mouse Caecum in Fasted vs. Fed Mice
Description	Bacterial extracellular vesicles (BEVs) produced by members of the intestinal microbiota can contribute not only to digestion but also mediate microbe-host cell communication via the transfer of functional biomolecules to mammalian host cells. An unresolved question is what host factors and conditions influence BEV cargoes and how do they impact on host cell function? To address this question, we analysed and compared the proteome of BEVs released by the major human gastrointestinal tract (GIT) symbiont Bacteroides thetaiotaomicron (Bt) in vivo in fed versus fasted animals using nano-liquid chromatography with tandem mass spectrometry (LC-MSMS). Among the proteins whose abundance was negatively affected by fasting, nine of ten proteins of the serine protease family, including the regulatory protein dipeptidyl peptidase-4 (DPP-4), were significantly decreased in BEVs produced in the GIT of fasted animals. Strikingly, in ex-tracellular vesicles produced by the intestinal epithelium of the same fasted mice, the proteins with the most increased abundance were serine protease inhibitors (serpins). Together, these findings suggest a dynamic interaction between GI bacteria and the host. Additionally, they indicate a regulatory role for the host in determining the balance be-tween bacterial serine proteases and host serpins exported in bacterial and host extra-cellular vesicles.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:12:27.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Regis Stentz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482); NCBI TaxID: 226186;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 08:26:19	ID requested
⏵ 1	2025-05-25 16:12:28	announced

10.3390/ijms26094080;

Stentz R, Jones E, Gul L, Latousakis D, Parker A, Brion A, Goldson AJ, Gotts K, Carding SR, Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors. Int J Mol Sci, 26(9):(2025) [pubmed]

submitter keyword: LC-MSMS,Extraccellular vesicles, Bacteroides thetaiotaomicron

Simon R Carding
contact affiliation	Quadram Institute Research Park, Rosalind Franklin Rd, Norwich NR4 7UQ
contact email	simon.carding@quadram.ac.uk
lab head
Regis Stentz
contact affiliation	Quadram Institute
contact email	regis.stentz@quadram.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062737

PRIDE project URI

• PRIDE
  1. PXD062737
     1. Label: PRIDE project
     2. Name: Comparative LC-MSMS Analysis of Extracellular Vesicles Extracted from Mouse Caecum in Fasted vs. Fed Mice