⮝ Full datasets listing

PXD062730

PXD062730 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAbout the effects of true to life polyethylene terephthalate nanoparticles on macrophages
DescriptionPlastics are emerging pollutants of great concern. Macroplastics degrade into microplastics and nanoplastics, which can accumulate in living organisms with still poorly known consequences. Nanoplastics being particulate pollutants, they are handled in animal organisms by scavenger cells such as macrophages, which are important players in the immune system. Polyethylene terephthalate is one of these plastics of concern, as it is widely used in food packaging where it releases nanoparticles. We have thus undertaken a study on the effects of true-to-life polyethylene terephthalate nanoparticles prepared from water bottles on macrophages. To this purpose, we used a combination of proteomics and targeted validation experiments. Proteomics showed important adaptive changes in the proteome in response to exposure to polyethylene terephthalate nanoparticles. These changes affected for example mitochondrial, cytoskeletal and lysosomal proteins, but also proteins implicated in immune functions. Validation experiments showed that many of these changes were homeostatic, with no induced oxidative stress and no gross perturbation of the mitochondrial function. However, polyethylene terephthalate nanoparticles induced endoplasmic reticulum stress and disturbed the immune functions of macrophages. We indeed observed a slight pro-inflammatory response (1.5-fold increase in TNF secretion). We also observed a decrease in the response to bacterial stimulation (1.6 decrease in IL-6 secretion). We also observed a 20 percent decrease in the expression of important proteins involved in immune responses such as TLR2, TLR7 or collectin 12, and a two-fold decrease in the production of lysozyme. This suggests that macrophages having ingested polyethylene terephthalate nanoparticles are less efficient in their immune functions.
HostingRepositoryPRIDE
AnnounceDate2025-04-09
AnnouncementXMLSubmission_2025-04-09_01:11:52.905.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD062730
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHélène Diemer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-08 06:23:52ID requested
12025-04-09 01:11:53announced
Publication List
10.1039/D4EN01063A;
10.6019/PXD062730;
Keyword List
submitter keyword: gel plugs, nanoplastic particle,macrophage, Mouse, nanoLC-MS/MS
Contact List
Sarah Cianferani
contact affiliationLaboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg,CNRS, IPHC UMR 7178, 67087 Strasbourg, France
contact emailsarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliationLSMBO
contact emailhdiemer@unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD062730
PRIDE project URI
Repository Record List
[ + ]