PXD062726

PXD062726 is an original dataset announced via ProteomeXchange.

Title	Conserved function of a RasGEF-mediated pathway in the metabolic compensation of the circadian clock
Description	Metabolic compensation of the circadian clock ensures endogenous timing across a broad range of nutrient conditions, enabling organisms to adapt efficiently to recurrent environmental changes, even during nutrient scarcity. In this study, we have identified a novel clock-controlled gene, rasgef (Rat Sarcoma Guanine Nucleotide Exchange Factor), that plays a crucial role in modulating the circadian clock under starvation conditions in the circadian model organism Neurospora crassa. The gene product, RasGEF – a nucleotide exchange factor for the small G-protein RAS2P (Rat Sarcoma 2 Protein) – displays glucose-dependent phosphorylation and localization. We show that deletion of rasgef hinders metabolic compensation of the circadian clock to glucose-depleted conditions and disrupts the rhythmic expression of the output gene ccg2. Furthermore, we demonstrate in osteosarcoma cells that period of the mammalian clock is also compensated across a wide range of extracellular glucose levels and adaptation of the clock to glucose-starved conditions depends on the RasGEF homologue SOS1 (Son of Sevenless 1) and its downstream signaling component ERK (Extracellular Signal-Regulated Kinase). Our results suggest a conserved role of RasGEF-mediated signaling in the maintenance of circadian rhythm under glucose-limited conditions.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-26_17:23:49.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aladár Pettkó-Szandtner
SpeciesList	scientific name: Neurospora crassa; NCBI TaxID: NEWT:5141;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 05:20:57	ID requested
⏵ 1	2025-10-26 17:23:50	announced

10.1111/febs.70122;

S, á, rk, á, ny O, Sz, ő, ke A, Pettk, ó, -Szandtner A, K, á, lm, á, n E É, Brunner M, Gy, ö, ngy, ö, si N, K, á, ldi K, Conserved function of a RasGEF-mediated pathway in the metabolic compensation of the circadian clock. FEBS J, 292(20):5335-5354(2025) [pubmed]

submitter keyword: RasGEF

Neurospora crassa

SOS1

circadian rhythm

metabolic compensation

RAS pathway

cAMP

ERK

BMAL1

Zsuzsanna Darula
contact affiliation	Single Cell Omics Advanced Core Facility - Hungarian Centre of Excellence for Molecular Medicine Budapesti út 9. | 6728 | Szeged | Hungary
contact email	darula.zsuzsanna@brc.hu
lab head
Aladár Pettkó-Szandtner
contact affiliation	Laboratory of Proteomics Research Biological Research Centre, Szeged
contact email	aladar@brc.hu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062726

PRIDE project URI

• PRIDE
  1. PXD062726
     1. Label: PRIDE project
     2. Name: Conserved function of a RasGEF-mediated pathway in the metabolic compensation of the circadian clock