PXD062705

PXD062705 is an original dataset announced via ProteomeXchange.

Title	Membrane proteome analysis of sorted splenic CD4+ T cells from SRBCs-immunized Rabggta-icKO, Ggps1-icKO and WT mice
Description	In order to explore the effects of Rabggta or Ggps1 on the membrane proteome of T cells, sorted CD4+ T cells of SRBCs-immunized Rabggta-icKO, Ggps1-icKO and their WT mice were subjected to membrane protein extraction with standard protocol. Take 50 μg protein and diluted to 100 μL water. Then add 5 μL (0.25 μg/μL) trpsin enzyme and incubate in a 37℃ constant temperature incubator for 16 hours for protein digestion. Then samples were subjected to Nano LC-MS/MS analysis. The raw MS files were analyzed and searched against target protein database based on the species of the samples using MaxQuant. The parameters were set as follows: the protein modifications were carbamidomethylation (C) (fixed), oxidation (M) (variable), Acetyl (N-term) (variable); the enzyme specificity was set to trypsin; the maximum missed cleavages were set to 2; the precursor ion mass tolerance was set to 20 ppm, and MS/MS tolerance was 20 ppm. Only high confident identified peptides were chosen for downstream protein identification analysis. Gene ontology (GO) terms were used to obtain and classify the function information of the DEPs.
HostingRepository	iProX
AnnounceDate	2025-04-07
AnnouncementXML	Submission_2025-04-08_00:13:35.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiao Jiang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 00:13:22	ID requested
⏵ 1	2025-04-08 00:13:35	announced

Dataset with its publication pending

submitter keyword: Membrane proteome analysis, CD4+ T cell, SRBC, Rabggta, Ggps1

Lai Wang
contact affiliation	Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College
contact email	wanglai@pumcderm.cams.cn
lab head
Jiao Jiang
contact affiliation	Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College
contact email	jiangjiaocsu@163.com
dataset submitter

iProX dataset URI