PXD062689

PXD062689 is an original dataset announced via ProteomeXchange.

Title	Candida albicans infection suppresses Lipopolysaccharide or Pseudomonas aeruginosa stimulated murine bone marrow derived macrophage (BMDM) responses
Description	Candida albicans is a commensal fungi which populates most healthy individuals’ microflora, but can turn opportunistic in immunocompromised individuals. This opportunistic pathogen can cause severe disease and is linked with high rates of mortality. With limited therapeutic options and increasing resistance to antifungals, novel treatment strategies for C. albicans infections is paramount. Macrophages are a key immune cell which mount an immediate response to C. albicans infection. Macrophages physically sequester C. albicans through phagocytosis, trapping the pathogen in a harsh environment. Additionally, macrophages mount a signalling response, alerting other immune cells to the site of infection for quick removal. Here we utilise a discovery screen approach using DIA based total proteomics to describe murine bone marrow derived macrophage (BMDM) response to C. albicans infection as well as in response to co-infection with gram-negative bacterial plasma membrane component lipopolysaccharide (LPS) or live gram-negative bacteria Pseudomonas aeruginosa. We found C. albicans induced a surprisingly muted response in BMDMs compared to LPS or P. aeruginosa. Despite small measurable activation of BMDMs on single infection, upon co-infection with LPS or P. aeruginosa, C. albicans significantly suppressed BMDM proteome landscape. Additionally, C. albicans selectively suppressed BMDM secreted IL-6 and IL-12p40 cytokine response to P. aeruginosa. Thus, we describe C. albicans significant suppressive capabilities in host innate immune responses.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_16:10:43.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christa Baker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Candida albicans (Yeast); NCBI TaxID: NEWT:5476;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-07 10:38:57	ID requested
⏵ 1	2026-03-16 16:10:44	announced

10.1038/s41598-026-39429-z;

Baker CP, Laba S, Warner J, Shepherd K, Wilson HM, Arthur JSC, Candida albicans infection suppresses lipopolysaccharide or Pseudomonas aeruginosa stimulated murine bone marrow derived macrophage (BMDM) responses. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: proteomics,bone marrow derived macrophages, co-infection, P. aeruginosa infection, C. albicans infection

J Simon C Arthur
contact affiliation	Cell Signalling and Immunology, School of life Sciences, University of Dundee
contact email	j.s.c.arthur@dundee.ac.uk
lab head
Christa Baker
contact affiliation	University of Dundee
contact email	cbaker001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD062689

PRIDE project URI

• PRIDE
  1. PXD062689
     1. Label: PRIDE project
     2. Name: Candida albicans infection suppresses Lipopolysaccharide or Pseudomonas aeruginosa stimulated murine bone marrow derived macrophage (BMDM) responses