PXD062684

PXD062684 is an original dataset announced via ProteomeXchange.

Title	Spatial tissue proteomics analysis of laser capture microdisscted intestinal crypt compartment
Description	Crypt hyperplasia is a key feature of celiac disease and various other small intestinal inflammatory conditions. By undertaking mass spectrometry-based tissue proteomics of the gut epithelial crypt zone, we found that active celiac disease is characterized by a strong interferon-γ (IFN-γ) signal. This signal, hallmarked by increased expression of MHC molecules, coincides with a markedly reduced expression of proteins associated with fatty acid metabolism. We observed similar crypt hyperplasia and proteomic alterations in wild-type mice treated with IFN-γ, but not in mice that lack the IFN-γ receptor in gut epithelial cells. IFN-γ is thus a driver of crypt hyperplasia in celiac disease by acting directly on crypt epithelial cells. This dataset contains proteomics analysis of crypt compartment samples isolated by laser capture microdissection small intestinal crypt compartment from C57/Bl6 mice injected i.p. with 0-1-2-4-6 or 9 doses interferon gamma every 8 hour.
HostingRepository	PRIDE
AnnounceDate	2025-08-08
AnnouncementXML	Submission_2025-08-08_03:10:09.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jorunn Stamnaes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-07 07:23:05	ID requested
⏵ 1	2025-08-08 03:10:10	announced

Dataset with its publication pending

submitter keyword: mouse, intestine, interferon-gamma, crypt hyperplasia

Jorunn Stamnæs
contact affiliation	Proteomics Core Facility, University of Oslo and Oslo University Hospital
contact email	jorunn.stamnas@medisin.uio.no
lab head
Jorunn Stamnaes
contact affiliation	University of Oslo Rikshospitalet
contact email	jorunn.stamnas@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD062684

PRIDE project URI

• PRIDE
  1. PXD062684
     1. Label: PRIDE project
     2. Name: Spatial tissue proteomics analysis of laser capture microdisscted intestinal crypt compartment