PXD062643

PXD062643 is an original dataset announced via ProteomeXchange.

Title	High Heme Resistance in Serratia plymuthica: Adaptive Mechanisms and Possible Role in Aedes aegypti Midgut Homeostasis
Description	Mosquitoes of the genus Aedes are primary vectors of arboviruses such as dengue, Zika, and chikungunya1. Their ability to transmit pathogens is intrinsically linked to their hematophagous behavior, which presents metabolic challenges, particularly during blood digestion2,3. One major challenge is the oxidative stress generated by heme, the prosthetic group of hemoglobin, which catalyzes the formation of reactive oxygen species (ROS)4. Excess heme can lead to cellular damage through lipid peroxidation and protein oxidation, necessitating specialized detoxification mechanisms in mosquitoes5,6.7. During blood digestion, heme concentrations in the mosquito midgut can exceed 10 mM, creating a highly oxidative environment that selects for resistant microbial species8. The midgut of A. aegypti hosts a diverse microbiota that plays a vital role in digestion, nutrient acquisition, and immune regulation9,10. Among these microbial inhabitants, bacteria of the genus Serratia are of particular interest due to their dominance in this oxidative environment11. As a Gram-negative, facultatively anaerobic bacterium, S. plymuthica exhibits resistance to oxidative stress, suggesting that it may play a role in heme homeostasis and redox balance within the mosquito midgut. Genomic analyses reveal that S. plymuthica encodes robust oxidative stress-response systems, including superoxide dismutase (sodA), catalases (katA/G), and glutathione S-transferases, which neutralize ROS and mitigate heme toxicity12. While mosquitoes have evolved antioxidant mechanisms, such as catalase induction, to mitigate heme toxicity, midgut bacteria like S. plymuthica may synergistically contribute to redox homeostasis. For instance, heme-peroxidases in mosquitoes regulate bacterial populations by modulating ROS levels and mucin crosslinking, creating a low-immunity zone that supports microbiota proliferation13. Conversely, Serratia species can internalize heme or produce antioxidant molecules, indirectly protecting the mosquito from oxidative damage while enhancing their own survival12,14,15. Such interactions are critical for maintaining microbial homeostasis and influencing vector competence, as dysbiosis in the midgut microbiota alters Plasmodium and arbovirus infections. Understanding the physiological and molecular adaptations of S. plymuthica to heme-induced oxidative stress is crucial for unraveling its survival strategies and potential role in mosquito-microbiota interactions. The midgut of A. aegypti presents a highly dynamic and oxidative environment, particularly following a blood meal, where bacterial symbionts must withstand heme toxicity while maintaining their metabolic functions12,13. We hypothesize that S. plymuthica plays an active role in mitigating oxidative stress in the mosquito gut, which may impact microbial homeostasis and vector competence. In this study, we assess the growth and oxidative stress tolerance of S. plymuthica in culture media supplemented with varying concentrations of iron and heme, coupled with a comparative proteomic analysis to identify differentially expressed proteins and metabolic pathways. By highlighting stress-response proteins, such as universal stress proteins and ABC transporters, we elucidate the adaptive mechanisms that enable S. plymuthica to persist in the mosquito midgut. These findings not only expand our understanding of mosquito-microbiota interactions but also offer new perspectives on vector competence and oxidative homeostasis, with potential implications for novel vector control strategies and biotechnological applications.
HostingRepository	PRIDE
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-06_17:39:21.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francisco Lemos
SpeciesList	scientific name: Serratia plymuthica; NCBI TaxID: 82996;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-05 21:26:00	ID requested
⏵ 1	2025-07-06 17:39:21	announced

Machado SDH, Rodrigues RCC, Bertonceli MAA, Gaio AO, Petroceli-Mota G, Reis RS, Berbert-Molina MA, Silveira V, Lemos FJA, Midgut. Life (Basel), 15(6):(2025) [pubmed]

10.3390/life15060950;

submitter keyword: heme detoxification, proteomics, oxidative stress, Aedes aegypti,Serratia plymuthica, midgut microbiota, hemin tolerance

Francisco José Aves Lemos
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia Universidade Estadual do Norte Fluminense Darcy Ribeiro Av. Alberto Lamego 2000 Campos dos Goytacazes-RJ, Brazil 28013-602
contact email	franze@uenf.br
lab head
Francisco Lemos
contact affiliation	Northern Fluminense State University, Biotechnology Laboratory, Center for Biosciences and Biotechnology, Campos dos Goytacazes, RJ, 28013-602.
contact email	franze@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD062643

PRIDE project URI

• PRIDE
  1. PXD062643
     1. Label: PRIDE project
     2. Name: High Heme Resistance in Serratia plymuthica: Adaptive Mechanisms and Possible Role in Aedes aegypti Midgut Homeostasis